Exogenous addition of indole acetic acid and kinetin under nitrogen-limited medium enhances lipid yield and expression of glycerol-3-phosphate acyltransferase & diacylglycerol acyltransferase genes in indigenous microalgae: A potential approach for biodiesel production
| Autor: | Neha Chaurasia, Madan Kumar Mandal, Ng. Kunjarani Chanu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Glycerol
0106 biological sciences Environmental Engineering Antioxidant Nitrogen medicine.medical_treatment Biomass Bioengineering Chlorophyta 010501 environmental sciences medicine.disease_cause 01 natural sciences Phosphates chemistry.chemical_compound 010608 biotechnology Gene expression Microalgae medicine Diacylglycerol O-Acyltransferase Food science Waste Management and Disposal 0105 earth and related environmental sciences Indoleacetic Acids biology Renewable Energy Sustainability and the Environment Fatty Acids General Medicine Kinetin biology.organism_classification Lipids chemistry Biofuels Biodiesel production Yield (chemistry) Oxidative stress |
| Zdroj: | Bioresource Technology. 297:122439 |
| ISSN: | 0960-8524 |
| Popis: | In the present study, a combination of phytohormones (indole acetic acid and kinetin) was augmented in nitrogen-limited medium to achieve higher biomass and lipid yield in Graesiella emersonii NC-M1 and Chlorophyta sp. NC-M5. This condition was recorded with a 2.3- and 2.5-fold increase in biomass and lipid yield for Graesiella emersonii NC-M1 compared to the nitrogen-limited condition. Also, this condition showed a 1.6- and 1.08-fold increase in lipid yield and neutral lipid compared to the standard condition. Phytohormones addition also reduced oxidative damage caused by nitrogen-limitation and enhanced monounsaturated fatty acid content. Further, a 5.2- and 3.17-fold enhance in expression level of GPAT and DGAT genes were noticed under nitrogen-limited medium supplemented with phytohormones compared to control. |
| Databáze: | OpenAIRE |
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