Impact of the Type of Continuous Insulin Administration on Metabolism in a Diabetic Rat Model

Autor: A. Langlois, C. Peronet, Michel Pinget, Stéphanie Dal, Cynthia Sookhareea, E. Seyfritz, Elisa Maillard, Séverine Sigrist, Nathalie Jeandidier, Anaïs Schaschkow, Carole Mura, William Bietiger
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Blood Glucose
Male
medicine.medical_specialty
Article Subject
Liver cytology
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Insulin Glargine
030209 endocrinology & metabolism
Inflammation
medicine.disease_cause
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Diabetes Mellitus
Experimental
03 medical and health sciences
0302 clinical medicine
Endocrinology
Bolus (medicine)
Insulin Infusion Systems
Diabetes mellitus
Internal medicine
medicine
Animals
Hypoglycemic Agents
Insulin
lcsh:RC648-665
business.industry
Macrophages
Body Weight
Metabolism
medicine.disease
Rats
030104 developmental biology
Diabetes Mellitus
Type 1
Liver
Rats
Inbred Lew
medicine.symptom
business
Reactive Oxygen Species
Omentum
Immunostaining
Oxidative stress
Research Article
Zdroj: Journal of Diabetes Research, Vol 2016 (2016)
Journal of Diabetes Research
ISSN: 2314-6745
DOI: 10.1155/2016/8310516
Popis: Exogenous insulin is the only treatment available for type 1 diabetic patients and is mostly administered by subcutaneous (SC) injection in a basal and bolus scheme using insulin pens (injection) or pumps (preimplanted SC catheter). Some divergence exists between these two modes of administration, since pumps provide better glycaemic control compared to injections in humans. The aim of this study was to compare the impacts of two modes of insulin administration (single injections of long-acting insulin or pump delivery of rapid-acting insulin) at the same dosage (4 IU/200 g/day) on rat metabolism and tissues. The rat weight and blood glucose levels were measured periodically after treatment. Immunostaining for signs of oxidative stress and for macrophages was performed on the liver and omental tissues. The continuous insulin delivery by pumps restored normoglycaemia, which induced the reduction of both reactive oxygen species and macrophage infiltration into the liver and omentum. Injections controlled the glucose levels for only a short period of time and therefore tissue stress and inflammation were elevated. In conclusion, the insulin administration mode has a crucial impact on rat metabolic parameters, which has to be taken into account when studies are designed.
Databáze: OpenAIRE
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