Formulation of magneto-responsive hydrogels from dually cross-linked polysaccharides: Synthesis, tuning and evaluation of rheological properties
| Autor: | Lenka Vítková, Lenka Musilová, Eva Achbergerová, Roman Kolařík, Miroslav Mrlík, Kateřina Korpasová, Leona Mahelová, Zdenka Capáková, Aleš Mráček |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Polymers
Organic Chemistry magnetorheology Hydrogels General Medicine smart hydrogels hyaluronan Schiff base hydrodynamic radius tissue engineering Catalysis Computer Science Applications Inorganic Chemistry Polysaccharides Physical and Theoretical Chemistry Hyaluronic Acid Rheology Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 23; Issue 17; Pages: 9633 |
| Popis: | Smart hydrogels based on natural polymers present an opportunity to fabricate responsive scaffolds that provide an immediate and reversible reaction to a given stimulus. Modulation of mechanical characteristics is especially interesting in myocyte cultivation, and can be achieved by magnetically controlled stiffening. Here, hyaluronan hydrogels with carbonyl iron particles as a magnetic filler are prepared in a low-toxicity process. Desired mechanical behaviour is achieved using a combination of two cross-linking routes-dynamic Schiff base linkages and ionic cross-linking. We found that gelation time is greatly affected by polymer chain conformation. This factor can surpass the influence of the number of reactive sites, shortening gelation from 5 h to 20 min. Ionic cross-linking efficiency increased with the number of carboxyl groups and led to the storage modulus reaching 10(3) Pa compared to 10(1) Pa-10(2) Pa for gels cross-linked with only Schiff bases. Furthermore, the ability of magnetic particles to induce significant stiffening of the hydrogel through the magnetorheological effect is confirmed, as a 10(3)-times higher storage modulus is achieved in an external magnetic field of 842 kA.m(-1). Finally, cytotoxicity testing confirms the ability to produce hydrogels that provide over 75% relative cell viability. Therefore, dual cross-linked hyaluronan-based magneto-responsive hydrogels present a potential material for on-demand mechanically tunable scaffolds usable in myocyte cultivation. project OP RDE Junior Grants of TBU in Zlin [CZ.02.2.69/0.0/0.0/19_073/0016941]; TBU in Zlin [IGA/CPS/2022/001]; Ministry of Education, Youth and Sports of the Czech Republic-DKRVO [RP/CPS/2022/003]; Ministry of Education, Youth and Sports of the Czech Republic project-DKRVO [RP/CPS/2022/001] |
| Databáze: | OpenAIRE |
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