Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina
| Autor: | D H Marticorena, Ana M. Vigliocco, R. F. Ramondino, Klaus Nielsen, C. Gorchs, D. Gall, Gabriel Briones, Patricia S. Silva Paulo, E. Bissi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Swine Clinical Biochemistry Immunology Argentina Enzyme-Linked Immunosorbent Assay Brucella Veterinary Immunology Brucellosis Serology Antigen Direct agglutination test parasitic diseases medicine Animals Immunology and Allergy Serologic Tests Swine Diseases biology Swine brucellosis biology.organism_classification medicine.disease Virology Herd Brucella suis |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 7:828-831 |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.7.5.828-831.2000 |
| Popis: | An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|