Hydrogen peroxide is involved in lymphocyte activation mechanisms to induce angiogenesis

Autor: Martin Monte, Davel L, E Sacerdote de Lustig
Rok vydání: 1997
Předmět:
Male
Cancer Research
CIENCIAS MÉDICAS Y DE LA SALUD
OXYGEN FREE RADICALS
Angiogenesis
T-Lymphocytes
TBARS
Antineoplastic Agents
Medicina Clínica
Oxidative phosphorylation
LYMPHOCYTES
Lymphocyte Activation
Thiobarbituric Acid Reactive Substances
ANGIOGENESIS
Oncología
SUPEROXIDE DISMUTASE
Ciencias Biológicas
Superoxide dismutase
Lipid peroxidation
Mice
chemistry.chemical_compound
Biología Celular
Microbiología
PUBLICACIONES
In vivo
Animals
HYDROGEN PEROXIDE
SACERDOTE INVESTIGADORA
chemistry.chemical_classification
Mice
Inbred BALB C
Reactive oxygen species
Neovascularization
Pathologic
biology
Superoxide Dismutase
Chemistry
TOMOUR
Hydrogen Peroxide
T lymphocyte
Catalase
Molecular biology
Oxidative Stress
Oncology
Biochemistry
Doxorubicin
biology.protein
CATALASE
Lipid Peroxidation
CIENCIAS NATURALES Y EXACTAS
Spleen
Zdroj: European Journal of Cancer. 33:676-682
ISSN: 0959-8049
DOI: 10.1016/s0959-8049(96)00506-0
Popis: T-lymphocytes from tumour-bearing mice are able to trigger the angiogenic cascade. Since it is known that tumour growth produces reactive oxygen species (ROS), the aim of this study was to evaluate the role of hydrogen peroxide (H202) on the activation of lymphocytes and their induction of this vascular response. Studies on lymphocytes, stimulated in vitro by ROS to induce angiogen- esis, showed that only the enzyme catalase (CAT) could block the activation. The incubation of nor- mal lymphocytes with H202 stimulated these cells to induce angiogenesis. The administration of H202 or an oxidative stress-producing drug (doxorubicin) to normal mice activated in viva angio- genesis. In tumour-bearing mice, high levels of lipid peroxidation products were observed in the spleen, but not in the liver or kidney. Moreover, when the ROS scavenger enzyme activities (super- oxide dismutase (SDM) and CAT) were determined, we observed low CAT activity in normal spleens, reflected in a high SDM/CAT ratio, when compared to liver or kidney values. We also showed an increasing value of the SDM/CAT ratio with tumour growth. These results strongty suggest that H202 could be involved in the mechanisms of lymphocyte activation and their induction of angiogenesis during tumour growth. Fil: Monte, M.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina Fil: Davel, L. E.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina Fil: Sacerdote de Lustig, Eugenia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Unidad documental simple
Databáze: OpenAIRE
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