Intermolecular transfer of aminoacylated adenosine towards chemical aminoacylation of tRNA

Autor:	Hiroshi Jinmei, Hiroshi Abe, Yoshihiro Ito, Mingzhe Liu, Koji Fukuzono
Rok vydání:	2007
Předmět:	Adenosine Stereochemistry Oligonucleotide Oligonucleotides RNA Aminoacylation DNA General Medicine Acceptor chemistry.chemical_compound RNA Transfer Biochemistry chemistry Transfer RNA medicine Transfer RNA Aminoacylation medicine.drug
Zdroj:	Nucleic Acids Symposium Series. 51:365-366
ISSN:	1746-8272 0261-3166
DOI:	10.1093/nass/nrm183
Popis:	We synthesized two oligonucleotides: one is DNA conjugated with adenosine derivative at 5' terminus as a donor, another RNA phosphorothioated at 3' terminus as an acceptor. By hybridization of the two oligonuleotides, the adenosine derivative was transferred from the donor DNA to the acceptor RNA. In this study, O, O'-isopropylidene-protected adenosine derivative and aminoacylated adenosine were successfully transferred from the donor to acceptor RNA. By use of tRNA instead of the acceptor RNA, the chemical aminoacylation of tRNA by unnatural amino acid is expected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::400c399ac4cd8bf8d59b6908337e7407 https://doi.org/10.1093/nass/nrm183 Zobrazit plný text záznamu