Development of Injectable Polydactyly-Derived Chondrocyte Sheets
| Autor: | Takumi Takahashi, Masahiko Watanabe, Eri Okada, Miki Maehara, Eriko Toyoda, Masato Sato, Shiho Wasai, Ryoka Uchiyama, Tadashi Akamatsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular Pathology medicine.medical_specialty Cell Survival Cell Gene Expression cell sheet Cell Count Osteoarthritis Catalysis Chondrocyte Article Flow cytometry Immunophenotyping minimally invasive treatment Inorganic Chemistry lcsh:Chemistry Chondrocytes medicine Animals Humans Regeneration Viability assay Physical and Theoretical Chemistry cartilage regeneration Molecular Biology lcsh:QH301-705.5 Spectroscopy medicine.diagnostic_test Tissue Scaffolds Chemistry Organic Chemistry Histology General Medicine Articular cartilage damage medicine.disease Immunohistochemistry Computer Science Applications Transplantation Polydactyly osteoarthritis medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 Biomarkers |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 3198, p 3198 (2021) International Journal of Molecular Sciences Volume 22 Issue 6 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | We are conducting a clinical study of the use of allogeneic polydactyly-derived chondrocyte sheets (PD sheets) for the repair of articular cartilage damage caused by osteoarthritis. However, the transplantation of PD sheets requires highly invasive surgery. To establish a less invasive treatment, we are currently developing injectable fragments of PD sheets (PD sheets-mini). Polydactyly-derived chondrocytes were seeded in RepCell™ or conventional temperature-responsive inserts and cultured. Cell counts and viability, histology, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), and flow cytometry were used to characterize PD sheets-mini and PD sheets collected from each culture. To examine the effects of injection on cell viability, PD sheets-mini were tested in four experimental conditions: non-injection control, 18 gauge (G) needle, 23G needle, and syringe only. PD sheets-mini produced similar amounts of humoral factors as PD sheets. No histological differences were observed between PD sheets and PD sheets-mini. Except for COL2A1, expression of cartilage-related genes did not differ between the two types of PD sheet. No significant differences were observed between injection conditions. PD sheets-mini have characteristics that resemble PD sheets. The cell viability of PD sheets-mini was not significantly affected by needle gauge size. Intra-articular injection may be a feasible, less invasive method to transplant PD sheets-mini. |
| Databáze: | OpenAIRE |
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