No difference in small bowel microbiota between patients with irritable bowel syndrome and healthy controls
Autor: | Katherina Zakikhany, Elin Lundin, Aldona Dlugosz, Lars Engstrand, Greger Lindberg, Björn Winckler, Weimin Ye, Gunnar Sandström |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Adult
Male medicine.medical_specialty Adolescent Firmicutes Veillonella medicine.disease_cause Gastroenterology Article Irritable Bowel Syndrome Young Adult Internal medicine Intestine Small medicine Prevotella Cluster Analysis Humans Irritable bowel syndrome Principal Component Analysis Multidisciplinary biology Streptococcus Microbiota Fusobacteria Biodiversity Middle Aged biology.organism_classification medicine.disease stomatognathic diseases Fusobacterium Case-Control Studies Metagenome Enterotype Female |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep08508 |
Popis: | Several studies have indicated that colonic microbiota may exhibit important differences between patients with irritable bowel syndrome (IBS) and healthy controls. Less is known about the microbiota of the small bowel. We used massive parallel sequencing to explore the composition of small bowel mucosa-associated microbiota in patients with IBS and healthy controls. We analysed capsule biopsies from the jejunum of 35 patients (26 females) with IBS aged 18-(36)-57 years and 16 healthy volunteers (11 females) aged 20-(32)-48 years. Sequences were analysed based on taxonomic classification. The phyla with the highest total abundance across all samples were: Firmicutes (43%), Proteobacteria (23%), Bacteroidetes (15%), Actinobacteria (9.3%) and Fusobacteria (7.0%). The most abundant genera were: Streptococcus (19%), Veillonella (13%), Prevotella (12%), Rothia (6.4%), Haemophilus (5.7%), Actinobacillus (5.5%), Escherichia (4.6%) and Fusobacterium (4.3%). We found no difference among major phyla or genera between patients with IBS and controls. We identified a cluster of samples in the small bowel microbiota dominated by Prevotella, which may represent a common enterotype of the upper small intestine. The remaining samples formed a gradient, dominated by Streptococcus at one end and Escherichia at the other. |
Databáze: | OpenAIRE |
Externí odkaz: |
načítá se...