DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
| Autor: | A C Olson, D Billen |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Cell Membrane Permeability
DNA Repair DNA synthesis Cell Survival Semiconservative replication DNA repair Chinese hamster ovary cell Deoxyribonucleotides DNA replication Polysorbates DNA Deoxycytidine Monophosphate Articles Cell Biology Biology Cell Line Polyethylene Glycols Cell biology chemistry.chemical_compound chemistry Biochemistry Cell culture Replicon |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.69.3.732 |
| Popis: | We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations. |
| Databáze: | OpenAIRE |
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