Geno/cytotoxicty and Apoptotic Properties of Phenolic Compounds from the Seeds of Dorema Glabrum Fisch. C.A
| Autor: | Morteza Eskandani, Elmira Dadizadeh, Hamed Hamishehkar, Jaleh Barar, Hossein Nazemiyeh |
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| Rok vydání: | 2017 |
| Předmět: |
Cytotoxicity
Pharmaceutical Science Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Annexin Botany medicine lcsh:QH301-705.5 IC50 lcsh:R5-920 General Medicine Molecular biology Phenolic compounds Comet assay Dorema glabrum lcsh:Biology (General) chemistry Phytochemical Apoptosis Genotoxicity Growth inhibition lcsh:Medicine (General) Research Article |
| Zdroj: | BioImpacts, Vol 4, Iss 4, Pp 191-198 (2014) BioImpacts : BI |
| ISSN: | 2228-5652 2228-5660 |
| DOI: | 10.15171/bi.2014.019 |
| Popis: | Introduction: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anticancer potency of bioactive compounds from the seeds of Dorma glabrum. Methods: Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds. Results: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 µg/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments. Conclusion: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum. |
| Databáze: | OpenAIRE |
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