Functional analysis of the stress response element and its role in the multistress response of Saccharomyces cerevisiae
| Autor: | Janet Treger, Kevin McEntee, Thomas R. Magee |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae
Genes Fungal Biophysics Biochemistry chemistry.chemical_compound Galactokinase Transcription (biology) Genes Reporter Gene Expression Regulation Fungal Escherichia coli Nucleotide Promoter Regions Genetic Molecular Biology Gene Transcription factor Genetics chemistry.chemical_classification biology Base Sequence Oligonucleotide Cell Biology biology.organism_classification Yeast chemistry Genes Bacterial Mutation Xenobiotic Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 243(1) |
| ISSN: | 0006-291X |
| Popis: | TheDDR2gene ofSaccharomyces cerevisiaeis a multistress response gene whose transcription is rapidly and strongly induced by a diverse array of xenobiotic agents, and environmental and physiological conditions. The multistress response of this gene requires the pentanucleotide, 5′CCCCT, (C4T; STRE ( ST ress R esponse E lement)) and the zinc-finger transcription factors, Msn2p and Msn4p. A 51bp oligonucleotide (oligo 31/32) containing two STREs from theDDR2promoter region was previously shown to direct heat shock activation of alacZreporter gene. In this work we demonstrate that the same element conferred a complete multistress response to anE. coli galKreporter gene introduced into yeast cells. A variant oligonucleotide in which both the STRE spacing and neighboring sequences were altered responded to the same spectrum of stresses, while substitution of nucleotides within the pentanucleotide completely abolished the multi-stress response. These results directly demonstrate that STREs are not only necessary but are sufficient for mediating a transcriptional response to a surprisingly diverse set of environmental and physiological conditions. |
| Databáze: | OpenAIRE |
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