Interleukin-17-producing T-helper cells and related cytokines in human airways exposed to endotoxin
| Autor: | Ingemar Qvarfordt, Margaretha E. Smith, Carina Malmhäll, B Balder, Pernilla Glader, A Linden, Margareta Sjöstrand |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pulmonary and Respiratory Medicine
Neutrophils medicine.medical_treatment Receptor Tyrosine Kinase-like Orphan Receptors Flow cytometry Bronchoscopy Macrophages Alveolar medicine Humans RNA Messenger Orphan receptor medicine.diagnostic_test Effector business.industry Interleukins Interleukin-17 Interleukin Nuclear Receptor Subfamily 1 Group F Member 1 T lymphocyte Flow Cytometry Endotoxins Bronchoalveolar lavage Cytokine Immunology Th17 Cells Interleukin 17 business Bronchoalveolar Lavage Fluid |
| Zdroj: | European Respiratory Journal. 36:1155-1164 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.00170609 |
| Popis: | Previous studies on mouse models have indicated that interleukin (IL)-17 and IL-17-producing T-helper (Th) cells are important for pulmonary host defence against Gram-negative bacteria. Human correlates to these findings have not yet been demonstrated. The aim of the present study was to determine whether or not IL-17-producing Th cells are present and whether IL-17 and other Th17-associated cytokines are involved in the immunological response to endotoxin in human airways. Segmental exposure to endotoxin and contralateral exposure to vehicle were performed in the lungs of healthy volunteers, with subsequent bronchoalveolar lavage 12 or 24 h after exposure to study local changes in cytokines and inflammatory cells. Endotoxin exposure increased concentrations of IL-17, IL-22 and their downstream effector molecules, human β-defensin-2 and IL-8/CXC chemokine ligand 8, in bronchoalveolar lavage fluid. Th cells with the capacity to produce IL-17 were found among the bronchoalveolar lavage cells, and expression of IL-17 mRNA correlated with expression of the transcription factor, retinoic-acid-receptor-related orphan receptor C variant 2. Moreover, endotoxin increased the numbers of neutrophils, macrophages and IL-17-producing T-cells, as well as the concentration of the Th17-regulating cytokines, IL-21 and IL-23. In conclusion, IL-17-producing Th cells are present, and IL-17, as well as other Th17-associated cytokines, is involved in the immunological response to endotoxin in human airways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|