Susceptibility of a Fetal Tongue Cell Line Derived from Bighorn Sheep to Five Serotypes of Bluetongue Virus and its Potential for the Isolation of Viruses
| Autor: | Anthony E. Castro, Steven R. Montague, David A. Jessup, Jill F. Dotson, James R. DeForge |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
0301 basic medicine
Serotype Virus Cultivation 040301 veterinary sciences 030106 microbiology Fluorescent Antibody Technique Animals Wild Biology medicine.disease_cause Virus Cell Line 0403 veterinary science 03 medical and health sciences Fetus Cytopathogenic Effect Viral Tongue Antigen medicine Animals Cytopathic effect Sheep General Veterinary symbols.heraldic_supporter 04 agricultural and veterinary sciences Mycoplasma Culicoides biology.organism_classification Virology Microscopy Electron Cell culture symbols Bluetongue virus Ovis canadensis |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 1:247-253 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063878900100310 |
| Popis: | A cell line (BHFTE) was derived from a tongue explant of a bighorn sheep fetus (Ovis canadensis nelsoni). The cells have been maintained through 23 serial passages, and the modal number of chromosomes was calculated to be 55. Monolayer cultures were shown to be susceptible to various viruses, including bluetongue virus (BTV). Of 5 BTV serotypes (2, 10, 11, 13, and 17) tested, each produced a cytopathic effect (CPE) on initial passage at 33 C. A field isolate (serotype 10) of BTV from a black-tailed deer (Odocoileus hemionus columbianus) in its second passage in Vero-M cells also produced CPE when inoculated into BHFTE cells. Antigens of BTV were demonstrated by direct immunofluorescence in the cytoplasm of BHFTE cells inoculated with homogenates of chicken embryos injected with clinical specimens from a domestic sheep and an Arabian oryx (Oryx gazella leucoryx). A suspension of BTV-infected gnats (Culicoides spp.) produced CPE and BTV-specific fluorescence on the first passage in cells inoculated with a suspension of blood from sheep experimentally infected with BTV. Additionally, selected bovine viruses induced CPE in the cells. The cell line, which is free of mycoplasma and bovine viral diarrhea virus contamination, may be useful in diagnostic medicine and research involving the ruminant species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|