Blood serum proteins and the mineralization of bone ground substance
| Autor: | Walther Lipp |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
chemistry.chemical_classification
Bone Development Histology Bone decalcification Osteoid Ground substance Albumin Osteoblast Blood Proteins Cell Biology Mineralization (biology) Bone and Bones Rats Medical Laboratory Technology Blood serum medicine.anatomical_structure chemistry Biochemistry Transferrin medicine Animals Molecular Biology |
| Zdroj: | Histochemie. 9:339-353 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/bf00305817 |
| Popis: | Direct tracing experiments with fluorochrome-labeled homologous blood serum show that certain serum components are taken up by the bone substance of young and adult rats. These proteins are concentrated and incorporated into the organic matrix while it is being formed. They retain their fluorescent label for long periods of time and withstand histological fixation and decalcification. In experimental rickets, no labeled serum protein is seen to be incorporated into the uncalcified rachitic osteoid. Its uptake begins, however, concomitantly with the onset of mineralization during the healing period. The results of experiments are interpreted in terms of a calcium-carrying serum protein being complexed and precipitated by osteoblast products to form an essential component of apposition zones which, possibly, initiates nucleation and the subsequent steps of calcification. Fractionation experiments to define the serum component(s) involved in this process, will be continued. So far, they have resulted in a fraction containing albumin, α1-macroglobulin, transferrin, and haptoglobin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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