Morphological analysis of leucocyte transmigration in the pleural cavity

Autor: Ruifeng Teng, I Iida, Xu Zhao, Katsunori Sasaki, Kazuhiko Asanuma, Naoko Ogiwara, Kohei Johkura, Li Cui, Yasumitsu Okouchi
Rok vydání: 2003
Předmět:
Lipopolysaccharides
Male
Pathology
medicine.medical_specialty
Histology
Immunoglobulins
Macrophage-1 Antigen
Vascular Cell Adhesion Molecule-1
Integrin alpha4beta1
Mice
chemistry.chemical_compound
Mucoproteins
Cell Movement
Leukocytes
Addressin
medicine
Animals
VCAM-1
Microscopy
Immunoelectron
Cell adhesion
Molecular Biology
Ecology
Evolution
Behavior and Systematics
Mice
Inbred ICR
ICAM-1
biology
Cell adhesion molecule
Membrane Proteins
Epithelial Cells
Original Articles
Cell Biology
Intercellular Adhesion Molecule-1
Immunohistochemistry
Lymphocyte Function-Associated Antigen-1
Fibronectins
Specific Pathogen-Free Organisms
Platelet Endothelial Cell Adhesion Molecule-1
Fibronectin
Endothelial stem cell
chemistry
Antigens
Surface
biology.protein
Pleura
Anatomy
E-Selectin
Cell Adhesion Molecules
Mesothelial Cell
Developmental Biology
Zdroj: Journal of Anatomy. 203:391-404
ISSN: 1469-7580
0021-8782
Popis: The role that pleural mesothelial cells play in leucocyte transmigration into the pleural cavity was investigated in lipopolysaccharide-stimulated mice. Changes in mesothelial cell morphology and changes in expression of adhesion molecules on mesothelial cells and leucocytes were analysed by light microscopy, immunohistochemistry, transmission electron microscopy (TEM) and immuno-scanning electron microscopy (immuno-SEM). After stimulation, the mesothelial cells separated completely from one another before leucocyte penetration across the mesothelial layer occurred. These changes occurred primarily in the immediate vicinity of ribs, where a large number of leucocytes accumulated. Immuno-SEM showed that the expression of intercellular adhesion molecule-1 (ICAM-1) on the parietal pleural mesothelial cells was significantly up-regulated by lipopolysaccharide stimulation, and that of vascular cell adhesion molecule-1 (VCAM-1) was induced. Both were restricted to the microvilli of the mesothelial cells. By contrast, expression of intercellular adhesion molecule-2 (ICAM-2), platelet/endothelial cell adhesion molecule-1 (PECAM-1), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), endothelial leucocyte adhesion molecule-1 (ELAM-1), peripheral node addressin (PNAd) and fibronectin were not detected. Lymphocyte function associated antigen-1 (LFA-1), macrophage-1 molecule (Mac-1) and very late appearing antigen-4 (VLA-4), all ligands of ICAM-1 and VCAM-1, were present on the transmigrated neutrophils and macrophages. These findings demonstrate that the immediate vicinity of ribs is a source of leucocyte migration into the pleural space.
Databáze: OpenAIRE
Externí odkaz: