Isolation and Molecular Identification of Fungi with Magnesite Enrichment Potential from KÜMAŞ Quarries in Turkey
Autor: | Medine Gulluce, Selma Sezen, Mehmet Karadayi, Selin Dogan, Taha Yasin Koc, Burak Alaylar |
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Přispěvatelé: | Belirlenecek |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
18S rDNA Bacteria Calcium pollutants fungi isolation ITS-PCR 030106 microbiology Areas 010501 environmental sciences Raw material Pulp and paper industry Isolation (microbiology) 01 natural sciences Microbiology magnesite enrichment 03 medical and health sciences chemistry.chemical_compound chemistry Earth and Planetary Sciences (miscellaneous) Environmental Chemistry 18s rdna 0105 earth and related environmental sciences General Environmental Science Magnesite Molecular identification |
Zdroj: | Geomicrobiology Journal. 37:618-622 |
ISSN: | 1521-0529 0149-0451 |
Popis: | Magnesite is an important raw material used in various industrial applications, especially the production of high-temperature resistant materials. Due to its high reactant nature, magnesite ore is not found in pure form and it contains a great variety of pollutants such as calcium compounds, which restrict its use when exceeding 1% of the ore. Thus, the development of efficient strategies for the removal of pollutants remains a crucial step for magnesite utilization. In this regard, our present work was conducted to isolate and identify active fungal strains that remove calcium pollutants without changing the main magnesium content of the ore. For this aim, magnesite ore samples were collected from two quarries (Turanocagi and Ortaocak) of KuMAS Magnesite Inc. and fungal isolation studies were done by using the ore's flora. Active isolates were chosen according to their CaCO(3)and MgCO(3)dissolving capabilities and identified by using conventional light microscopy and molecular characterization techniques. 71 fungal isolates were obtained from the isolation step and 14 of them were chosen as active isolates that solve calcium compounds while not affecting the magnesium component. The data of the microscopic examination and 18S rDNA gene sequence analysis showed that 14 active strains with magnesite enrichment potential grouped inAspergillus alliaceus(3),Aspergillus flavus(2),Aspergillus leporis(1),Aspergillus nomius(1),Fusarium tricinctum(2),Penicillium chrysogenum(1) andPenicillium sp.(4). |
Databáze: | OpenAIRE |
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