The method for integrating dual-color fluorescence colocalization and single molecule photobleaching technology on the theophylline sensing platform
| Autor: | Shi Gang Liu, Yu He, Wenli Gao, Xing-Bo Shi, Dong Zhang, Zhaodi Fu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Aptamer Clinical Biochemistry Dual-color fluorescence colocalization Nanotechnology 010501 environmental sciences 01 natural sciences 03 medical and health sciences Theophylline Single molecule photobleaching Molecule lcsh:Science 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Bioelectronics Biosensing Colocalization Method Article Fluorescence Single Molecule Imaging Photobleaching Medical Laboratory Technology lcsh:Q Image J Biosensor |
| Zdroj: | MethodsX MethodsX, Vol 7, Iss, Pp 101155-(2020) |
| ISSN: | 2215-0161 |
| DOI: | 10.1016/j.mex.2020.101155 |
| Popis: | • Smart usage of single molecule photobleaching technology and dual-color fluorescence colocalization is of critical importance for exploiting the sensing platform. Here, we provide the detailed protocols related to the article “A split aptamer sensing platform for highly sensitive detection of theophylline based on dual-color fluorescence colocalization and single molecule photobleaching” (published online by Biosensors and Bioelectronics) (Liu et al., 2020). The protocols contain: (1) how to clean the slides; (2) how to prepare the probe and detection sample; (3) Single molecule imaging; 4) Data processing by using the Image J. Finally, we used a simple model to confirm the feasibility of the method for integrating dual-color fluorescence colocalization and single molecule photobleaching technology on the theophylline sensing platform. • A simple, ultrasensitive method for the detection of theophylline. • The method is easily comprehensible. • Both strategy formulation and data processing are simple, learnability, and highly reproducible. Graphical abstract Image, graphical abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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