Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes*
| Autor: | Susan M. Barns, Christy C. Grow, Lori Merrill, Cheryl R. Kuske, John Dunbar |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Clostridium perfringens Yersinia pestis Virulence Biology medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction Pathology and Forensic Medicine Microbiology law.invention law Genetics medicine Francisella tularensis Pathogen Phylogeny Soil Microbiology Polymerase chain reaction DNA Primers Aerosols Phylogenetic tree Pathogenic bacteria Sequence Analysis DNA Forensic Medicine 16S ribosomal RNA biology.organism_classification Bacillus anthracis Environmental Monitoring |
| Zdroj: | Journal of Forensic Sciences. 51:548-558 |
| ISSN: | 1556-4029 0022-1198 |
| DOI: | 10.1111/j.1556-4029.2006.00131.x |
| Popis: | Bacterial species with high DNA sequence similarity to pathogens could affect the specificity of assays designed to detect biological threat agents in environmental samples. The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yersinia pestis and their closely related species, was determined for a large collection of soil and aerosol samples. Polymerase chain reaction (PCR) and gene sequencing were used using group-specific 16S rRNA primers to identify pathogens and related species, and pathogen-specific virulence genes. Close relatives of B. anthracis (B. cereus group species) were detected in 37% of the soils and 25% of the aerosol samples. The B. anthracis protective antigen (pag) gene or a close homolog was detected in 16 of these samples. For the other three pathogen groups, the frequency of detection was much lower, and none of the samples were positive with both the phylogenetic and virulence gene primer sets. |
| Databáze: | OpenAIRE |
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