Site-specific analysis of advanced glycation end products in plasma proteins of type 2 diabetes mellitus patients
| Autor: | Andrej Frolov, Matthias Blüher, Uta Greifenhagen, Ralf Hoffmann |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Glycation End Products
Advanced 0301 basic medicine medicine.medical_specialty Arginine Lysine Plasma protein binding Proteomics Tandem mass spectrometry Sensitivity and Specificity Biochemistry Analytical Chemistry 03 medical and health sciences Tandem Mass Spectrometry Glycation Internal medicine Protein Interaction Mapping medicine Humans Binding Sites 030102 biochemistry & molecular biology Chemistry Reproducibility of Results Type 2 Diabetes Mellitus Blood Proteins Blood proteins 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 Biomarkers Protein Binding |
| Zdroj: | Analytical and Bioanalytical Chemistry. 408:5557-5566 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-016-9651-4 |
| Popis: | Advanced glycation end products (AGEs) are posttranslational modifications formed non-enzymatically from the reaction of carbohydrates and their degradation products with proteins. Accumulation of AGEs is associated with the progression of severe diabetic complications, for example, and elevated tissue levels of AGEs might even predict these pathologies. As AGE formation is often site-specific, mapping of these modification sites may reveal more sensitive and specific markers than the global tissue level. Here, 42 AGE modifications were identified in a bottom-up proteomic approach by tandem mass spectrometry, which corresponded to 36 sites in 22 high to medium abundant proteins in individual plasma samples obtained from type 2 diabetes mellitus (T2DM) patients with long disease duration (>10 years). Major modifications were glarg (11 modification sites) and carboxymethylation (5) of arginine and formylation (8), acetylation (7), and carboxymethylation (7) of lysine residues. Relative quantification of these sites in plasma samples obtained from normoglycemic individuals (n = 47) and patients with T2DM being newly diagnosed (n = 47) or of medium (2–5 years, n = 20) and long disease duration (>10 years, n = 20) did not reveal any significant differences. |
| Databáze: | OpenAIRE |
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