Evaluation of the OSIRIS video reader system for disk diffusion susceptibility test reading
| Autor: | Elena Loza, Rafael Cantón, F. Baquero, B. Sánchez del Saz, Miguel Sánchez |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Veterinary medicine Susceptibility testing Inhibition zone Lactams Staphylococcus media_common.quotation_subject Video Recording Microbial Sensitivity Tests Sensitivity and Specificity Methicillin resistance beta-Lactam Resistance Diffusion Enterobacteriaceae Reference Values video reader automatic system Reading (process) Image Processing Computer-Assisted Humans Medicine Prospective Studies Disk diffusion susceptibility test media_common Vancomycin resistance biology business.industry Reproducibility of Results Vancomycin Resistance General Medicine biology.organism_classification Anti-Bacterial Agents Infectious Diseases Reference values Methicillin Resistance Disk diffusion Osiris business Enterococcus |
| Zdroj: | Clinical Microbiology and Infection. 7(7):352-357 |
| ISSN: | 1198-743X |
| DOI: | 10.1046/j.1198-743x.2001.00274.x |
| Popis: | Objective The objective of this study was to compare the performance of the OSIRIS video-assisted reading system for disk diffusion susceptibility testing with conventional manual reading. Methods Prospectively collected clinical isolates ( n = 119) and isolates with well-characterised resistant mechanisms, including extended-spectrum (ESBL) or inhibitor-resistant TEM (IRT) β-lactamases producing Enterohacteriaceae (80), methicillin-resistant Staphylococcus aureus (MRSA) (16) and vancomycin-resistant enterococci (14) were studied using the National Committee for Clinical Laboratory Standards disk-diffusion technique. The OSIRIS reading (inhibition zone in mm) was compared with manual reading (reference value). Results Essential agreement (≤3 mm discrepancy with manual reading) was 91.6% in routine isolates and 94.8% in those with well-characterised resistant mechanisms, respectively. Overall agreement for susceptibility testing interpretation was slightly higher in the former (95.5%) than in the latter (93.2%) group. The presence of ESBL enzymes enhanced variations of measurements due to synergy among amoxicillin—clavulanate and cephalosporins, as a consequence of closer disk placement. The poor growth characteristic of enterococci affected the video reading; on the other hand, there was a high performance with MRSA isolates. Combining all interpretative results, 4.1% minor, 1.0% major and 2.8% very major errors were observed. Conclusion The OSIRIS system is a useful tool for the reading and interpretation of inhibition zone sizes in disk diffusion susceptibility testing. |
| Databáze: | OpenAIRE |
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