Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase
Autor: | Beatriz Guilarte Maresma, Rubén Cuervo Fernandez, Alfredo Eduardo Maiorano, Maria Filomena de Andrade Rodrigues, Elda Sabino da Silva, Boris Gutarra Castillo |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
chemistry.chemical_classification
Fructosyltransferase activity Aspergillus oryzae Mutant UV irradiation Biology biology.organism_classification Microbiology Enzyme assay chemistry.chemical_compound Industrial Microbiology Enzyme chemistry Biochemistry Mutagenesis Fructooligosaccharides Extracellular biology.protein β-fructofuranosidase Fructosyltransferase Sodium dodecyl sulfate Mycelium Research Paper |
Zdroj: | Brazilian Journal of Microbiology, Volume: 41, Issue: 1, Pages: 186-195, Published: MAR 2010 Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.41 n.1 2010 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
Popis: | Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with β-fructofuranosidase activity values relative to the parent culture between 140 - 190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities. Three mutants named IPT-745, IPT-746 and IPT-748 showed the highest amount of mycelium activity whose values increased 1.5 - 1.8 fold, compared with the parent strain. It was found that more than 55% of total enzyme activity (mycelium- plus extracellular- activity) from these strains was detected in the mycelium fraction. Only one mutant, IPT-747, exceeded the amount of extracellular enzyme exhibited by the parent strain (1.5 times). This mutant also showed the highest value of total fructosyltransferase activity. |
Databáze: | OpenAIRE |
Externí odkaz: |