Characterization of bacteriocinogenic Enterococcus isolates from wild and laboratory rabbits for the selection of autochthonous probiotic strains in Tunisia
| Autor: | A. Rehaiem, Mohamed Salah Abbassi, T. Najar, S. Lengliz, N. Ben Chehida |
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| Rok vydání: | 2021 |
| Předmět: |
Tunisia
Virulence Factors Virulence Animals Wild Microbial Sensitivity Tests medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Feces 03 medical and health sciences Antibiotic resistance Animals Laboratory Ampicillin Drug Resistance Bacterial medicine Animals Pathogen 030304 developmental biology 0303 health sciences biology 030306 microbiology Probiotics Pathogenic bacteria General Medicine biology.organism_classification Enterococcus durans Anti-Bacterial Agents Enterococcus Rabbits Bacteria Biotechnology medicine.drug |
| Zdroj: | Journal of Applied Microbiology. 131:1474-1486 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | Aim The objective of this study was to characterize lactic acid bacteria (LAB) from rabbits to be used as potential autochthonous probiotic. Methods and results Fifteen faecal samples were collected from wild and laboratory rabbits. One hundred and eight isolates were collected and tested for their inhibitory power against eight pathogenic bacteria. Among them, 43 Enterococcus isolates were able to inhibit at least one pathogen. Enterocine genes entA, entB and entP were detected in 14, 17 and 22 isolates, respectively. These isolates were tested for their antibiotic susceptibility and genes encoding virulence factors. Relevant phenotypes of antibiotic resistance were observed especially for ampicillin, vancomycin and linezolid. The following virulence genes were detected (number of positive isolates): hyl (5), esp (8), gelE (30), agg (2), ace (21), efa (6), CylLL/s (5), cob (26), cpd (32) and ccf (33). Five isolates were considered as safe and showed tolerance to both acid and bile salt. Conclusion Bacteriocinogenic enterococci isolates from rabbits may show relevant resistance phenotypes and virulence factors. In addition, one Enterococcus durans isolate presents promising autochthonous probiotic candidate. Significance and impact of the study This study reveals interesting properties for E. durans isolate and supports their utilization as autochthonous probiotic in rabbit husbandry. |
| Databáze: | OpenAIRE |
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