Microphotonic control of single molecule fluorescence correlation spectroscopy using planar optofluidics

Autor:	Dongliang Yin, Holger Schmidt, Aaron R. Hawkins, Evan J. Lunt, Anjan Barman
Rok vydání:	2007
Předmět:	Materials science Silicon photonics business.industry Resolution (electron density) Physics::Optics Fluorescence correlation spectroscopy Single-molecule experiment Atomic and Molecular Physics and Optics Optofluidics Fluorescence spectroscopy Optics Microscopy Spectroscopy business
Zdroj:	Optics Express. 15:7290
ISSN:	1094-4087
Popis:	We demonstrate the implementation of fluorescence correlation spectroscopy (FCS) on a chip. Full planar integration is achieved by lithographic definition of sub-picoliter excitation volumes using intersecting solid and liquid-core optical waveguides. Concentration dependent measurements on dye molecules with single molecule resolution are demonstrated. Theoretical modeling of the FCS autocorrelation function in microstructured geometries shows that the FCS behavior can be controlled over a wide range by tailoring the micro-photonic environment. The ability to perform correlation spectroscopy using silicon photonics without the need for free-space microscopy permits implementation of numerous diagnostic applications on compact planar optofluidic devices.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fe887d9d7b3c121f6d3536ee5a8f820 https://doi.org/10.1364/oe.15.007290 Zobrazit plný text záznamu