Activation of the cellular oncogene c-erbB by ltr insertion: Molecular basis for induction of erythroblastosis by avian leukosis virus
| Autor: | Wynne G. Lewis, Yuen Kai T Fung, Hsing Jien Kung, Lyman B. Crittenden |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
viruses
Locus (genetics) Biology General Biochemistry Genetics and Molecular Biology Virus Deoxyribonuclease EcoRI chemistry.chemical_compound Retrovirus Erythroblast ErbB Operon Animals Coding region Cloning Molecular Poultry Diseases Avian Leukosis Virus Base Sequence Oncogene DNA Restriction Enzymes Oncogenes biology.organism_classification Virology Avian Leukosis chemistry DNA Viral Chickens DNA |
| Zdroj: | Cell. 33:357-368 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(83)90417-8 |
| Popis: | Avian leukosis virus (ALV), a slowly oncogenic retrovirus, induces in chickens a variety of neoplasms, including lymphoid leukosis and erythroblastosis. In lymphoid leukosis, a cellular oncogene, c-myc, is activated by the insertion of ALV LTR. We provide evidence that ALV utilizes a similar mechanism in erythroblastosis induction by activating a different cellular oncogene, c-erbB. We report the isolation, from leukemic erythroblast DNA, of a clone that represents the viral-cell junction fragment and carried the ALV LTR and part of the c-erbB locus. Restriction and sequence analyses reveal that the LTR is located upstream from the erbB coding region and is oriented in the same transcriptional direction; such a structure would be compatible with the promoter-insertion type of activation. Our findings provide a molecular explanation for the multipotency of slowly oncogenic retroviruses. |
| Databáze: | OpenAIRE |
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