Unique patterns of mitochondrial genes, transcripts and proteins in different male-sterile cytoplasms of Daucus carota
| Autor: | Manfred Mehring-Lemper, Günter Wricke, Rupert Scheike, Axel Brennicke, Ernst Gerold |
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| Rok vydání: | 1992 |
| Předmět: |
Genetics
Mitochondrial DNA Mitochondrial translation Cytoplasmic male sterility General Medicine Biology Mitochondrion Restriction fragment Chloroplast DNA health services administration biology.protein Restriction fragment length polymorphism Agronomy and Crop Science Gene health care economics and organizations Biotechnology |
| Zdroj: | Theoretical and Applied Genetics. 83:419-427 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/bf00226529 |
| Popis: | Restriction fragment analysis of mitochondrial and chloroplast DNAs from a brown anther and a petaloid cytoplasmic male-sterile (cms) line revealed unique patterns for each cms line distinct from those of normal fertile cytoplasms, but identical restriction fragments for all chloroplast DNAs. The restauration of fertility through the introduction of nuclear restorer genes had no effect on the overall mitochondrial DNA (mtDNA) structure. The genomic environment and transcription patterns of several mitochondrial genes differ between cms and normal cytoplasms, while no difference has so far been detected between cms and the corresponding fertility-restored lines in mitochondrial DNAs and mRNAs. Mitochondrial translation products analysed by in-organello synthesized proteins revealed a number of polypeptides unique to each cytoplasm. Most prominent is a 17-kDa polypeptide that is present in the brown anther cms line but not in fertile mitochondria. Synthesis of this protein was not visibly affected by fertility restauration. The different cms phenotypes in carrot are thus associated with extensive and unique mtDNA rearrangements and distinct alterations in transcription and translation patterns. |
| Databáze: | OpenAIRE |
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