Cell Death Triggered by a Putative Amphipathic Helix of Radish mosaic virus Helicase Protein Is Tightly Correlated With Host Membrane Modification
| Autor: | Yukari Okano, Shigetou Namba, Yugo Kitazawa, Ryo Iwai, Yasuyuki Yamaji, Masayoshi Hashimoto, Takuya Shiraishi, Tetsuya Yoshida, Kensaku Maejima, Kazuya Ishikawa, Ken Komatsu, Takuya Keima |
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| Rok vydání: | 2015 |
| Předmět: |
Hypersensitive response
Programmed cell death Physiology Recombinant Fusion Proteins Radish mosaic virus Comovirus Molecular Sequence Data Endoplasmic Reticulum Virus Replication Protein Structure Secondary Raphanus Necrosis Viral Proteins Genes Reporter Tobacco SIN3A Amino Acid Sequence Plant Diseases Cell Death biology Endoplasmic reticulum DNA Helicases RNA Intracellular Membranes General Medicine biology.organism_classification Cerulenin Cell biology Elicitor Plant Leaves Biochemistry Viral replication Mutagenesis Site-Directed Sequence Alignment Agronomy and Crop Science |
| Zdroj: | Molecular Plant-Microbe Interactions®. 28:675-688 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | Systemic necrosis is one of the most severe symptoms caused by plant RNA viruses. Recently, systemic necrosis has been suggested to have similar features to a defense response referred to as the hypersensitive response (HR), a form of programmed cell death. In virus-infected plant cells, host intracellular membrane structures are changed dramatically for more efficient viral replication. However, little is known about whether this replication-associated membrane modification is the cause of the symptoms. In this study, we identified an amino-terminal amphipathic helix of the helicase encoded by Radish mosaic virus (RaMV) (genus Comovirus) as an elicitor of cell death in RaMV-infected plants. Cell death caused by the amphipathic helix had features similar to HR, such as SGT1-dependence. Mutational analyses and inhibitor assays using cerulenin demonstrated that the amphipathic helix–induced cell death was tightly correlated with dramatic alterations in endoplasmic reticulum (ER) membrane structures. Furthermore, the cell death–inducing activity of the amphipathic helix was conserved in Cowpea mosaic virus (genus Comovirus) and Tobacco ringspot virus (genus Nepovirus), both of which are classified in the family Secoviridae. Together, these results indicate that ER membrane modification associated with viral intracellular replication may be recognized to prime defense responses against plant viruses. |
| Databáze: | OpenAIRE |
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