Stanniocalcin 1 Inhibits the Inflammatory Response in Microglia and Protects Against Sepsis-Associated Encephalopathy
| Autor: | Jucélia Jeremias Fortunato, Raquel Jaconi De Carli, Mariana Pereira de Souza Goldim, Evandro Cittadin, Fabricia Petronilho, Larissa Joaquim, Vijayasree V. Giridharan, Bianca Xavier de Farias, Sandra Bonfante, Amanda Della Giustina, Rafael Mariano de Bitencourt, Jaqueline S. Generoso, Lucineia Gainski Danielski, Maria Eduarda Fileti, Giselli Scaini, Tatiana Barichello, Gislaine T. Rezin, Naiana da Rosa, Silvia Resende Terra, Nicole Alessandra Engel |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Inflammation Pharmacology Toxicology medicine.disease_cause Neuroprotection Hippocampus Sepsis 03 medical and health sciences 0302 clinical medicine Medicine Animals Rats Wistar Neuroinflammation Cells Cultured Glycoproteins Microglia business.industry General Neuroscience Sepsis-Associated Encephalopathy medicine.disease Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Mitochondrial respiratory chain Neuroprotective Agents Encephalitis medicine.symptom business 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Neurotoxicity research. 39(2) |
| ISSN: | 1476-3524 |
| Popis: | Sepsis-associated encephalopathy is a serious consequence of sepsis, triggered by the host response against an infectious agent, that can lead to brain damage and cognitive impairment. Several mechanisms have been proposed in this bidirectional communication between the immune system and the brain after sepsis as neuroinflammation, oxidative stress, and mitochondrial dysfunction. Stanniocalcin-1 (STC-1), an endogen neuroprotective protein, acts as an anti-inflammatory and suppresses superoxide generation through induction of uncoupling proteins (UCPs) in the mitochondria. Here, we demonstrated a protective role of STC-1 on inflammatory responses in vitro, in activated microglia stimulated with LPS, and on neuroinflammation, oxidative stress, and mitochondrial function in the hippocampus of rats subjected to an animal model of sepsis by cecal ligation and puncture (CLP), as well the consequences on long-term memory. Recombinant human STC-1 (rhSTC1) suppressed the pro-inflammatory cytokine production in LPS-stimulated microglia without changing the UCP-2 expression. Besides, rhSTC1 injected into the cisterna magna decreased acute hippocampal inflammation and oxidative stress and increased the activity of complex I and II activity of mitochondrial respiratory chain and creatine kinase at 24 h after sepsis. rhSTC1 was effective in preventing long-term cognitive impairment after CLP. In conclusion, rhSTC1 confers significant neuroprotection by inhibiting the inflammatory response in microglia and protecting against sepsis-associated encephalopathy in rats. |
| Databáze: | OpenAIRE |
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