Distinct Genetic Diversity of Carbapenem-ResistantAcinetobacter baumanniifrom Colombian Hospitals
| Autor: | Federico Perez, Rafael Cantón, Marcela Perenguez, Cristhian Hernández-Gómez, Kevin Escandón-Vargas, Maria V. Villegas, Rosa del Campo, Mercedes Rodríguez-Baños, Cesar A. Arias, Christian Pallares, Adriana Correa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine Microbiology (medical) Epidemiology 030106 microbiology Immunology Microbial Sensitivity Tests Colombia Serogroup Microbiology beta-Lactam Resistance beta-Lactamases 03 medical and health sciences Antibiotic resistance Multiplex polymerase chain reaction Pulsed-field gel electrophoresis Humans Pharmacology Molecular Epidemiology Genetic diversity biology Molecular epidemiology Genetic Variation Gene Expression Regulation Bacterial Acinetobacter bacterial infections and mycoses biology.organism_classification Hospitals Anti-Bacterial Agents Clone Cells Electrophoresis Gel Pulsed-Field Carbapenems Multilocus sequence typing Multiplex Polymerase Chain Reaction Acinetobacter Infections Multilocus Sequence Typing |
| Zdroj: | Microbial Drug Resistance. 24:48-54 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2016.0190 |
| Popis: | The global success of multidrug-resistant Acinetobacter baumannii has been associated with the dissemination of a high-risk clone designated clonal complex (CC) 92B (Bartual scheme)/CC2P (Pasteur scheme), which is the most frequent genetic lineage in European, Asian, and North American carbapenem-resistant Acinetobacter isolates. In these isolates, carbapenem resistance is mainly mediated by β-lactamases encoded by blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and/or blaOXA-58-like genes. In this study, we characterized the population genetics of 121 carbapenem-resistant A. baumannii complex isolates recovered from 14 hospitals in seven cities in Colombia (2008–2010). Multiplex PCR was used to detect blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like genes. Molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PCR showed that 118 (97.5%) of the isolates were positive for both blaOXA-23-like and blaOXA-51-like genes, and three other isolates were only positive for blaOXA-51-like. PFGE identified 18 different pulsotypes, while MLST identified 11 different sequence types (STs), seven of which had not been previously described in Acinetobacter. None of the STs found in this study was associated with CC92B/CC2P. The most widespread STs in our isolates belonged to ST636 and their single-locus variants ST121/ST124/ST634 (CC636B) followed by STs belonging to CC110B. Our observations suggest a wide distribution of diverse A. baumannii complex clones containing blaOXA-23-like in Colombian hospitals (especially CC636B and CC110B) that differ from the high-risk clones commonly found in other regions of the world, indicating a distinct molecular epidemiology of carbapenem-resistant Acinetobacter spp. in Colombia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|