Identification of a second protein encoded by influenza C virus RNA segment 6
| Autor: | Seiji Hongo, Kiyoto Nakamura, Kanetsu Sugawara, Yasushi Muraki, Hidekazu Nishimura, Fumio Kitame |
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| Rok vydání: | 1994 |
| Předmět: |
Influenzavirus C
Genes Viral Immunoprecipitation RNA Splicing Recombinant Fusion Proteins M1 protein Molecular Sequence Data Biology Viral Matrix Proteins Viral Proteins Japan Virology Escherichia coli Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence chemistry.chemical_classification Messenger RNA Base Sequence RNA Genetic Variation Sequence Analysis DNA Fusion protein Molecular biology Amino acid Molecular Weight chemistry biology.protein RNA Viral Influenza C Virus |
| Zdroj: | The Journal of general virology. 75 |
| ISSN: | 0022-1317 |
| Popis: | Influenza C virus matrix protein (M1) is encoded by a spliced mRNA derived from RNA segment 6. Unspliced mRNA from this RNA segment, which has not been previously identified, can potentially encode a polypeptide that contains an additional 132 amino acids on the carboxy terminus of the M1 protein. Here the nucleotide sequences of RNA segment 6 of four influenza C strains, isolated in Japan between 1964 and 1988, were compared with the previously determined sequence of C/Ann Arbor/1/50. The results indicated that the deduced amino acid sequence of the carboxy-terminal 132 amino acid domain is conserved fairly well although it is more divergent than the M1 protein sequence. Examination of RNA segment 6-specific mRNAs also showed that unspliced mRNA is present, although in small quantities (approximately 13% of spliced mRNA), in influenza C virus-infected cells. To search for a polypeptide encoded by the unspliced mRNA, the extra carboxy-terminal domain was expressed in Escherichia coli as the glutathione S-transferase fusion protein, and rabbit immune serum was raised against the purified fusion protein. Immunoprecipitation experiments with this antiserum revealed that a previously unrecognized protein of apparent M(r) approximately 18,000, designated CM2, is synthesized in influenza C virus-infected cells. |
| Databáze: | OpenAIRE |
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