Immune response induced by oral administration with a Saccharomyces cerevisiae-based SARS-CoV-2 vaccine in mice
| Autor: | Shuangqin Li, Tong Gao, Yi Ren, Xin Lu, Han Lei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
COVID-19 Vaccines Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Saccharomyces cerevisiae Administration Oral EBY100/pYD1-RBD Bioengineering Biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Mice 0302 clinical medicine Immune system Oral administration Pandemic Animals Immune response Immunity Mucosal Immunity Cellular Mice Inbred BALB C Binding Sites Research Spike Protein COVID-19 biology.organism_classification QR1-502 Immunity Humoral Bacterial vaccine Universal technology platform 030104 developmental biology Immunology Spike Glycoprotein Coronavirus Female 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Microbial Cell Factories, Vol 20, Iss 1, Pp 1-10 (2021) Microbial Cell Factories |
| ISSN: | 1475-2859 |
| Popis: | Background The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the need to develop safe and effective vaccines with a top priority. Multiple vaccine candidates are under development, and several vaccines are currently available. Efforts need to be undertaken to counter the threat of the global COVID-19 pandemic. Results We generated a Saccharomyces cerevisiae (S. cerevisiae)-based SARS-CoV-2 vaccine, EBY100/pYD1-RBD, in which the full-length receptor binding domain (RBD) of the spike protein of SARS-CoV-2 was expressed on the surface of yeast. Mice vaccinated orally with unadjuvanted EBY100/pYD1-RBD could produce significant humoral and mucosal responses as well as robust cellular immune responses. Notably, EBY100/pYD1-RBD elicited a mixed Th1/Th2-type cellular immune response with a Th1-biased immune response in a mouse model. Conclusions Our findings highlight the importance of the RBD as a key target to design and develop vaccines against SARS-CoV-2 and provide evidence of oral administration of a S. cerevisiae-based SARS-CoV-2 vaccine eliciting significant immune responses. Most importantly, the S. cerevisiae surface display system can serve as a universal technology platform and be applied to develop other oral viral or bacterial vaccines. |
| Databáze: | OpenAIRE |
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