PvMSP8 as a Novel Plasmodium vivax Malaria Sero-Marker for the Peruvian Amazon
| Autor: | Katherine Garro, Elizabeth Villasis, Michael T. White, Katherine Torres, Dionicia Gamboa, Pamela Rodriguez, Anthony Gave, Paulo Manrique, Mitchel Guzman-Guzman, Niko Speybroeck, Joseph M. Vinetz, Angel Rosas-Aguirre, Jason Rosado |
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| Přispěvatelé: | HAL-SU, Gestionnaire, Universidad Peruana Cayetano Heredia (UPCH), Université Catholique de Louvain = Catholic University of Louvain (UCL), Malaria : parasites et hôtes - Malaria : parasites and hosts, Institut Pasteur [Paris] (IP), Santé publique : épidémiologie & sciences de l'information biomédicale (ED 393), Sorbonne Université (SU), Yale School of Medicine [New Haven, Connecticut] (YSM), This research was funded by: 'Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica' (FONDECYT/CONCYTEC), under the Contract 218-2015-FONDECYT, The International Centers of Excellence for Malaria Research (ICEMR) program (U19AI089681), NIH–USA, FOGARTY International Center (D43TW007120), NIH-USA. EV is supported by the CONCYTEC-FONDECYT-World Bank Group contract number E033-01-08-2018-FONDECYT/Banco Mundial-Programas de Doctorado en Áreas Estratégicas y Generales. ARA is a Postdoctoral Researcher of the Fonds de la Recherche Scientifique (FNRS, Belgium)., Institut Pasteur [Paris], Yale University School of Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:Medicine
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity law.invention Serology 0302 clinical medicine law [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Immunology and Allergy P. vivax antibodies purl.org/pe-repo/ocde/ford#3.03.09 [http] 0303 health sciences education.field_of_study biology Area under the curve 3. Good health Infectious Diseases Transmission (mechanics) [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology [SDV.IMM.IA] Life Sciences [q-bio]/Immunology/Adaptive immunology Cohort [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Biomarker (medicine) ELISA Antibody Microbiology (medical) PvMSP8 030231 tropical medicine Population malaria Antibodies 03 medical and health sciences parasitic diseases medicine Luminex education Molecular Biology [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity 030304 developmental biology General Immunology and Microbiology business.industry lcsh:R medicine.disease purl.org/pe-repo/ocde/ford#3.01.09 [https] Malaria Immunology biology.protein business |
| Zdroj: | Pathogens Volume 10 Issue 3 Pathogens, 2021, 10 (3), pp.282. ⟨10.3390/pathogens10030282⟩ Pathogens, Vol 10, Iss 282, p 282 (2021) CONCYTEC-Institucional Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica instacron:CONCYTEC Pathogens, MDPI, 2021, 10 (3), pp.282. ⟨10.3390/pathogens10030282⟩ |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens10030282 |
| Popis: | The measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house Plasmodium vivax Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to P. vivax (Pv) in the Peruvian Amazon. In a first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex in a cohort of 422 Amazonian individuals with known history of Pv exposure (monthly data of infection status by qPCR and/or microscopy over five months). Both serological responses were able to discriminate between exposed and non-exposed individuals in a good manner, with slightly higher performance of anti-PvMSP10 IgGs (area under the curve AUC = 0.78 [95% CI = 0.72–0.83]) than anti-PvMSP8 IgGs (AUC = 0.72 [95% CI = 0.67–0.78]) (p = 0.01). In a second evaluation, the analysis by ELISA of 1251 plasma samples, collected during a population-based cross-sectional survey, confirmed the good performance of anti-PvMSP8 IgGs for discriminating between individuals with Pv infection at the time of survey and/or with antecedent of Pv in the past month (AUC = 0.79 [95% CI = 0.74–0.83]). Anti-PvMSP8 IgG antibodies can be considered as a good biomarker of recent Pv exposure in low-moderate transmission settings of the Peruvian Amazon. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| Databáze: | OpenAIRE |
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