Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro
| Autor: | Camila Cantarelli, Alfredo Q. Antoniazzi, Luciana Maria Fontanari Krause, Gabriele Biavaschi da Silva, Alexandre Krause, Heloisa Einloft Palma, Flávio Desessards De La Corte, Miguel Gallio, Karin Erica Brass, Patrícia Wolkmer, Kalyne Bertolin, Julien Wergutz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
osteoartrite
Type II collagen Osteoarthritis MMP9 Matrix metalloproteinase lcsh:Agriculture Extracellular matrix medicine lcsh:Agriculture (General) cartilage Aggrecan IL-6 General Veterinary Chemistry Cartilage lcsh:S 0402 animal and dairy science agrecano 04 agricultural and veterinary sciences medicine.disease lcsh:S1-972 040201 dairy & animal science Molecular biology cartilagem osteoarthritis medicine.anatomical_structure Platelet-rich plasma 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology MMPs aggrecan Agronomy and Crop Science |
| Zdroj: | Ciência Rural v.49 n.7 2019 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Ciência Rural, Vol 49, Iss 7 Ciência Rural, Volume: 49, Issue: 7, Article number: e20180262, Published: 04 JUL 2019 |
| Popis: | In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group when the expressions of MMP9, MMP13, IL-6 and ACAN genes were evaluated (P |
| Databáze: | OpenAIRE |
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