Shedding light on toxicity of SARS-CoV-2 peptides in aquatic biota: A study involving neotropical mosquito larvae (Diptera: Culicidae)
| Autor: | Ives Charlie-Silva, Marilia Freitas Calmon, Cíntia Bittar, Christoph F.A. Vogel, Abraão Tiago Batista Guimarães, Guilherme Malafaia, Paulo R. S. Sanches, Paula Rahal, Juliana Moreira Mendonça-Gomes, Fernanda Neves Estrela, Eduardo Maffud Cilli, Rafael Nava Miceli, Mohamed Ahmed Ibrahim Ahmed |
|---|---|
| Přispěvatelé: | Universidade de São Paulo (USP), Instituto Federal Goiano, Universidade Estadual Paulista (UNESP), São José do Rio Preto, Assiut University, University of California, Universidade Federal de Goiás (UFG), Universidade Federal de Uberlância |
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Developmental toxicity Zoology Toxicology medicine.disease_cause Article Contamination medicine Animals Humans Pandemics Coronavirus Cholinesterase Invertebrate Larva biology SARS-CoV-2 fungi COVID-19 Water General Medicine biology.organism_classification Pollution Biota Culex quinquefasciatus Insects Culicidae Toxicity Spike Glycoprotein Coronavirus biology.protein Peptides |
| Zdroj: | Environmental Pollution (Barking, Essex : 1987) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1873-6424 |
| Popis: | Knowledge about how the COVID-19 pandemic can affect aquatic wildlife is still extremely limited, and no effect of SARS-CoV-2 or its structural constituents on invertebrate models has been reported so far. Thus, we investigated the presence of the 2019-new coronavirus in different urban wastewater samples and, later, evaluated the behavioral and biochemical effects of the exposure of Culex quinquefasciatus larvae to two SARS-CoV-2 spike protein peptides (PSPD-2002 and PSPD-2003) synthesized in our laboratory. Initially, our results show the contamination of wastewater by the new coronavirus, via RT-qPCR on the viral N1 gene. On the other hand, our study shows that short-term exposure (48 h) to a low concentration (40 μg/L) of the synthesized peptides induced changes in the locomotor and the olfactory-driven behavior of the C. quinquefascitus larvae, which were associated with increased production of ROS and AChE activity (cholinesterase effect). To our knowledge, this is the first study that reports the indirect effects of the COVID-19 pandemic on the larval phase of a freshwater invertebrate species. The results raise concerns at the ecological level where the observed biological effects may lead to drastic consequences. Graphical abstract Image 1 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect