Cleavage of type 2 adenovirus DNA by HaeIII endonuclease. I. Catalog of HaeIII fragments
Autor: | Reginald McParland, H. Mark Engelking, George D. Pearson |
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Rok vydání: | 1978 |
Předmět: |
Gel electrophoresis
Adenovirus DNA Molecular mass Base Sequence Genes Viral Haemophilus Chromosome Mapping DNA Restriction Enzymes Biology bacterial infections and mycoses EcoRI Endonuclease Cleavage (embryo) Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular biology HaeIII SmaI Adenoviridae Molecular Weight HaeIII endonuclease DNA Viral medicine bacteria Electrophoresis Polyacrylamide Gel medicine.drug |
Zdroj: | Biochimica et biophysica acta. 518(3) |
ISSN: | 0006-3002 |
Popis: | Tye 2 adenovirus DNA was divided into 14 fragments by sequential use of BamI, HsuI, SmaI, anc EcoRI endonuclease. Each fragment was purified by gel electrophoresis and subsequently cleaved with HaeIII endonuclease. From the number of fragments produced, we could calculate the number of HaeIII cleavage sites: there are a total of 187 sites. HaeIII sites were not randomly distributed along the adenovirus chromosome. Most sites were clustered in the G + C-rich left half of the chromosome. The sum of the molecular weights of the HaeIII fragments is 22.4 . 10(6), within 2 % of the molecular weight of adenovirus DNA (22.9 . 10(6). |
Databáze: | OpenAIRE |
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