MicroRNA-190 regulates FOXP2 genes in human gastric cancer
| Autor: | Xiao Liu, Wenzhuo Jia, Tao Yu, Zhu Zhang, Hua Yang, Gang Xiao, Qi An |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
RT-PCR OncoTargets and Therapy Western blotting CCK-8 03 medical and health sciences 0302 clinical medicine SGC7901 cell line Downregulation and upregulation microRNA Gastric mucosa medicine Pharmacology (medical) Luciferase Original Research migration assay Chemistry Transfection Molecular biology Blot 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure Oncology Cell culture transwell 030220 oncology & carcinogenesis targeted regulation |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| Popis: | Wen-Zhuo Jia,1 Tao Yu,1 Qi An,1 Hua Yang,1 Zhu Zhang,2 Xiao Liu,2 Gang Xiao1 1Department of General Surgery, 2Department of Gastroenterology, Beijing Hospital, Beijing, People’s Republic of China Objective: To investigate how microRNA-190 (miR-190) regulates FOXP2 genes in gastric cancer (GC) cell line SGC7901. Methods: We identified that miR-190 could target FOXP2 genes by using dual luciferase enzyme assay. Precursor fragment transfection of miR-190 was performed with GC cell line SGC7901 and human gastric mucosal cell line GES-1. miR-190 expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and FOXP2 protein expression was measured by Western blotting. Results: FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups. There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group. Therefore, it was assumed that miR-190 can target FOXP2 genes. Through RT-PCR verification, it was observed that the expression level of miR-190 was significantly higher in GC cell line SGC7901 than in human gastric mucosa cell line GES-1 after transfection with miR-190 mimics. The expression level of miR-190 was significantly higher in GES-1 cells than in SGC7901 cells after transfection with miR-190 inhibitors. Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells. Compared with blank, mimics control, and inhibitors control groups, the miR-190 mimics group showed significantly enhanced proliferation, migration, and invasion abilities, while miR-190 inhibitors group showed decreased abilities toward proliferation, migration, and invasion (P |
| Databáze: | OpenAIRE |
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