CHD3 and CHD4 recruitment and chromatin remodeling activity at DNA breaks is promoted by early poly(ADP-ribose)-dependent chromatin relaxation
| Autor: | Catherine Chapuis, Rebecca Smith, Sébastien Huet, Hafida Sellou, Gyula Timinszky |
|---|---|
| Přispěvatelé: | Institut de Génétique et Développement de Rennes ( IGDR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Centre National de la Recherche Scientifique ( CNRS ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Ludwig-Maximilians-Universität München, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Ludwig-Maximilians-Universität München (LMU), 14-1315, Worldwide Cancer Research, LP2017-11/2017, Magyar Tudományos Akadémia, 20161204883, Fondation ARC pour la Recherche sur le Cancer, FP7/2007-2013, Seventh Framework Programme, PCOFUND-GA-2013-609102, Research Executive Agency, PRESTIGE-2017-2-0042, Campus France, FP7-PEOPLE-2011-CIG, European Union, FK_02_16, Bayerisch-Französische Hochschulzentrum, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Poly Adenosine Diphosphate Ribose DNA damage [SDV]Life Sciences [q-bio] Biology Genome Integrity Repair and Replication Chromatin remodeling CHD1L Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine PARP1 Genetics Humans ComputingMilieux_MISCELLANEOUS [ SDV ] Life Sciences [q-bio] Adenosine diphosphate ribose DNA Breaks DNA Helicases Chromatin Assembly and Disassembly Chromatin 3. Good health Cell biology 030104 developmental biology chemistry CHD4 030217 neurology & neurosurgery DNA Mi-2 Nucleosome Remodeling and Deacetylase Complex Signal Transduction |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, 2018, 〈10.1093/nar/gky334〉 Nucleic Acids Research, 2018, 46 (12), pp.6087-6098. ⟨10.1093/nar/gky334⟩ Nucleic Acids Research, Oxford University Press, 2018, 46 (12), pp.6087-6098. ⟨10.1093/nar/gky334⟩ |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gky334〉 |
| Popis: | International audience; One of the first events to occur upon DNA damage is the local opening of the compact chromatin architecture, facilitating access of repair proteins to DNA lesions. This early relaxation is triggered by poly(ADP-ribosyl)ation by PARP1 in addition to ATP-dependent chromatin remodeling. CHD4 recruits to DNA breaks in a PAR-dependent manner, although it lacks any recognizable PAR-binding domain, and has the ability to relax chromatin structure. However, its role in chromatin relaxation at the site of DNA damage has not been explored. Using a live cell fluorescence three-hybrid assay, we demonstrate that the recruitment of CHD4 to DNA damage, while being poly(ADP-ribosyl)ation-dependent, is not through binding poly(ADP-ribose). Additionally, we show that CHD3 is recruited to DNA breaks in the same manner as CHD4 and that both CHD3 and CHD4 play active roles in chromatin remodeling at DNA breaks. Together, our findings reveal a two-step mechanism for DNA damage induced chromatin relaxation in which PARP1 and the PAR-binding remodeler activities of Alc1/CHD1L induce an initial chromatin relaxation phase that promotes the subsequent recruitment of CHD3 and CHD4 via binding to DNA for further chromatin remodeling at DNA breaks. |
| Databáze: | OpenAIRE |
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