The immunoregulatory and neuroprotective effects of human adipose derived stem cells overexpressing IL-11 and IL-13 in the experimental autoimmune encephalomyelitis mice
Autor: | Maryam Azimzadeh, Hossein Salehi, Mazdak Ganjalikhani Hakemi, Asma Eslami, Morteza Jafarinia, Mohammad Kazemi, Merat Mahmoodi, Noushin Amirpour |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Adult Encephalomyelitis Autoimmune Experimental Multiple Sclerosis medicine.medical_treatment Immunology Adipose tissue Immunomodulation 03 medical and health sciences Mice Young Adult 0302 clinical medicine medicine Immunology and Allergy Animals Humans Remyelination Pharmacology Interleukin-13 business.industry Multiple sclerosis Experimental autoimmune encephalomyelitis Stem-cell therapy medicine.disease Interleukin-11 Peptide Fragments Interleukin 11 Mice Inbred C57BL Adult Stem Cells Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Neuroprotective Agents Adipose Tissue 030220 oncology & carcinogenesis Interleukin 13 Female Myelin-Oligodendrocyte Glycoprotein business Stem Cell Transplantation |
Zdroj: | International immunopharmacology. 87 |
ISSN: | 1878-1705 |
Popis: | Multiple sclerosis (MS) is an inflammatory demyelination disease in the central nervous system (CNS) characterized by incomplete endogenous remyelination in the chronic phase. A shift of the balance between pro and anti-inflammatory cytokines is one of the important markers in the pathogenesis of MS. This study aimed to evaluate the effects of human adipose derived stem cells (hADSCs) overexpressing interleukin 11 and interleukin 13 (IL-11, 13-hADSCs) on the experimental autoimmune encephalomyelitis (EAE), an animal model of MS. 12 days after immunization of C57Bl/6 female mice with MOG35-55 and initial clinical symptoms appearance, the IL-11, 13-hADSCs were injected via the tail vein into the EAE mice. Then, the mice were sacrificed at 30 days post-immunization (DPI) and the spinal cords of experimental groups were extracted for histopathological and real-time RT-PCR studies. The results indicated that the clinical scores and mononuclear cells infiltration into the spinal cords of EAE mice were significantly reduced in mice treated with IL-11, 13-hADSCs. Likewise, the remyelination and oligodendrogenesis were significantly enhanced in the mentioned treatment group. Real-time results demonstrated that pro/anti-inflammatory cytokine genes expression was reversed in IL-11, 13-hADSCs treatment group in comparison to the untreated EAE group. Expression of IL-11 as a neurotrophic cytokine and IL-13 as an anti-inflammatory cytokine by hADSCs could increase the immunomodulatory and neuroprotective effects of hADSCs and be a powerful candidate in stem cell therapy for future treatment of MS. |
Databáze: | OpenAIRE |
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