Verapamil preferentially potentiatesin-vitro cytotoxicity of vincristine on malignant lymphoid cells
| Autor: | Nicolas V. Costea, Imtiaz A. Malik |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Vincristine Chronic lymphocytic leukemia Drug Resistance In vivo hemic and lymphatic diseases Acute lymphocytic leukemia Tumor Cells Cultured medicine Humans Leukemia-Lymphoma Adult T-Cell Lymphocytes Cytotoxicity Dose-Response Relationship Drug business.industry Lymphoblast Drug Synergism Hematology General Medicine medicine.disease Leukemia Lymphocytic Chronic B-Cell In vitro Verapamil Oncology Immunology Cancer research business medicine.drug |
| Zdroj: | Hematological Oncology. 10:225-231 |
| ISSN: | 1099-1069 0278-0232 |
| DOI: | 10.1002/hon.2900100314 |
| Popis: | Verapamil has been shown to overcome acquired drug resistance to vincristine in P388 leukemia both in vitro and in vivo. To study the selectivity of this action, the effect of addition of verapamil on the cytotoxicity of vincristine was studied using lymphocytes from eight patients with chronic lymphocytic leukemia (CLL), lymphoblasts from a T-acute lymphoblastic leukemia (T-ALL) cell line (GM 3639), and peripheral blood lymphocytes (PBL) from eight normal healthy volunteers. Using the differential staining cytotoxicity (DiSC) assay, we demonstrated that verapamil at 1 microM concentration potentiated the in-vitro cytotoxicity of vincristine on CLL and GM 3639 cells in concentrations of 0.04-0.25 micrograms/l. There was however, no enhancement of cytotoxicity noted against the control PBL. The data demonstrate that verapamil preferentially enhances the in-vitro cytotoxicity of vincristine on CLL and GM 3639 cells but no enhancement of cytotoxicity is seen against PBL. |
| Databáze: | OpenAIRE |
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