Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae
| Autor: | Lena Burri, David I Stapleton, Lynn Ramage, Ian E Gentle, Katherine Vascotto, Nickie C Chan, Trevor Lithgow, Traude H. Beilharz |
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| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Protein Conformation Translocase of the outer membrane Molecular Sequence Data Peripheral membrane protein Membrane Proteins Saccharomyces cerevisiae Cell Biology Biology Biochemistry Transmembrane protein Mitochondria Cell biology Mitochondrial Proteins Membrane protein Translocase of the inner membrane Outer membrane efflux proteins Virulence-related outer membrane protein family Amino Acid Sequence Molecular Biology Integral membrane protein |
| Zdroj: | Monash University |
| ISSN: | 1742-4658 1742-464X |
| DOI: | 10.1111/j.1742-4658.2006.05171.x |
| Popis: | Mitochondria evolved from a bacterial endosymbiont ancestor in which the integral outer membrane proteins would have been beta-barrel structured within the plane of the membrane. Initial proteomics on the outer membrane from yeast mitochondria suggest that while most of the protein components are integral in the membrane, most of these mitochondrial proteins behave as if they have alpha-helical transmembrane domains, rather than beta-barrels. These proteins are usually predicted to have a single alpha-helical transmembrane segment at either the N- or C-terminus, however, more complex topologies are also seen. We purified the novel outer membrane protein Om14 and show it is encoded in the gene YBR230c. Protein sequencing revealed an intron is spliced from the transcript, and both transcription from the YBR230c gene and steady-state level of the Om14 protein is dramatically less in cells grown on glucose than in cells grown on nonfermentable carbon sources. Hydropathy predictions together with data from limited protease digestion show three alpha-helical transmembrane segments in Om14. The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. |
| Databáze: | OpenAIRE |
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