Inhibition of the mixed lymphocyte culture by peritoneal exudate cells
| Autor: | R.B. Herberman, H. Kirchner, B.R. Fernbach |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
C57BL/6
Mice Inbred BALB C Cell-Free System biology Effector Macrophages Lymphocyte Immunology Exudates and Transudates Selective inhibition biology.organism_classification Microbiology Mice Inbred C57BL Mice medicine.anatomical_structure Precursor cell Peritoneal exudate medicine Animals Ascitic Fluid Cytotoxic T cell Lymphocyte Culture Test Mixed Spleen Mixed lymphocyte culture |
| Zdroj: | Cellular Immunology. 22:399-403 |
| ISSN: | 0008-8749 |
| Popis: | Inhibition of mixed lymphocyte cultures (MLC) by macrophages and by supernatants of short term cultured macrophages was assessed by incorporation of 3H-thymidine (TdRH3) and also by blast cell counts and by determination of cellmediated lympholysis. Peritoneal exudate cells (PEC) induced by thioglycollate, at concentrations >10%, inhibited all three parameters of MLC. Lower concentrations of PEC, and supernatants from cultured PEC, inhibited TdRH3 incorporation, but had no significant effect on blast cell counts or on generation of cytotoxic effector cells. Inhibition by the supernatants could be reversed by dialysis or by use of low specific activity TdRH3. These data indicate that macrophages can inhibit proliferative responses in MLC, but that this must be carefully distinguished from selective inhibition of TdRH3 incorporation. |
| Databáze: | OpenAIRE |
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