Simultaneous determination of Allium compounds (Propyl propane thiosulfonate and thiosulfinate) in animal feed using UPLC-MS/MS
| Autor: | Enrique Guillamón, Ángeles Jos, Remedios Guzmán-Guillén, Giorgiana M. Cătunescu, Ana Isabel Prieto Ortega, José M. de la Torre, Antonio Cascajosa-Lira, Ana Mª Cameán Fernández |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Ministerio de Ciencia, Innovación y Universidades (MICINN). España, Junta de Andalucía |
| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Animal feed Chemistry Food analysis Extraction (chemistry) Thiosulfonic Acids General Medicine Toxicology Tandem mass spectrometry Rotary evaporator Animal Feed Bioactive compounds Allium Solvent Response surface methodology UPLC-MS/MS Limit of Detection Tandem Mass Spectrometry Organosulfur compounds Thiosulfinate Chromatography High Pressure Liquid Food Science |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 1873-6351 |
| Popis: | Propyl-propane-thiosulfonate (PTSO) and Propyl-propane-thiosulfinate (PTS) are organosulfur compounds used to supplement the diet of livestock because of their beneficial effects on feed palatability, their antibacterial, anti-inflammatory, and antimethanogenic activities. Besides, antibiotic residues in the environment can be reduced by using these natural bioactive compounds. The objective of this study was to optimize the extraction parameters for the analysis of PTSO and PTS in feed matrices by performing a solid-liquid extraction and quantification by Ultra performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Optimization was performed using the Response Surface Methodology on a Box–Behnken experimental design, optimizing the following parameters: solvent:sample ratios and evaporation temperature set for the rotary evaporator. The method was validated for 3 concentration levels for both PTSO (100, 500, 1000 ng g−1) and PTS (500, 1150, 2300 ng g−1). The highest recoveries of PTSO and PTS were obtained using 12.5 mL of 100% acetonitrile, stirring for 15 min, and an evaporation temperature of 20 °C. The validated method was further applied to detect and quantify these compounds in different feed matrices. In conclusion, this is the first study to simultaneously analyze PTSO and PTS at low concentrations, employing a sensitive technique such as UPLC-MS/MS. Ministerio de Ciencia, Innovación y Universidades (RTC-2017-6199-2) Junta de Andalucía (AT-17-5323) |
| Databáze: | OpenAIRE |
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