Manzamine A as a novel inhibitor of herpes simplex virus type-1 replication in cultured corneal cells
| Autor: | Gautam R Bedadala, Khalid A. El Sayed, Jayavardhana R Palem, Shao-Chung V Hsia |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
viruses
Carbazoles Pharmaceutical Science Herpesvirus 1 Human medicine.disease_cause Virus Replication Antiviral Agents Virus Herpesviridae Article Analytical Chemistry Cell Line Cornea Alphaherpesvirinae Drug Discovery medicine Animals Aciclovir Pharmacology biology Organic Chemistry Eye infection biology.organism_classification Virology Porifera Herpes simplex virus Complementary and alternative medicine Viral replication Cell culture Molecular Medicine Rabbits medicine.drug |
| Zdroj: | Planta medica. 77(1) |
| ISSN: | 1439-0221 |
| Popis: | This study investigated the putative inhibitory effect of manzamine A on HSV-1 infection. Our results indicated that manzamine A effectively inhibited viral replication and infection in the cell line SIRC, a corneal cell line, at 1 µM. The existing anti-HSV-1 drug acyclovir was analyzed and showed a comparable activity at 50 µM. Plaque assays demonstrated that manzamine A reduced the release of infectious virus by 10 (11)-fold. RTPCR assays indicated that HSV-1 virion host shutoff (vhs) activity and ICP0 transcription were decreased by manzamine A treatment. These results bode well for the development of manzamines as potential leads to reduce viral infection in corneal cells and to prevent HSV-1-induced eye infections such as keratitis. |
| Databáze: | OpenAIRE |
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