Bacillus macyae sp. nov., an arsenate-respiring bacterium isolated from an Australian gold mine

Autor: Illo Christian Arnold. Streimann, Joanne M. Santini, Rachel N. vanden Hoven
Rok vydání: 2004
Předmět:
Bacillus
Acetates
chemistry.chemical_compound
RNA
Ribosomal
16S
Enzyme Stability
Anaerobiosis
Phylogeny
Soil Microbiology
Arsenite
chemistry.chemical_classification
biology
Nucleic Acid Hybridization
General Medicine
Electron acceptor
Hydrogen-Ion Concentration
RNA
Bacterial
Arsenate reductase
Biochemistry
Enzyme Induction
Arsenates
Oxidation-Reduction
DNA
Bacterial
Arsenites
Movement
Molecular Sequence Data
Ion Pumps
Microbiology
DNA
Ribosomal
Mining
Bacterial Proteins
Multienzyme Complexes
Ecology
Evolution
Behavior and Systematics
Bacillaceae
Nitrates
Arsenite Transporting ATPases
Arsenate
Australia
Membrane Proteins
Genes
rRNA
Sequence Analysis
DNA
Ribosomal RNA
biology.organism_classification
Bacillales
Molecular Weight
Protein Subunits
chemistry
Phenazines
Gentian Violet
Gold
Bacteria
Zdroj: International journal of systematic and evolutionary microbiology. 54(Pt 6)
ISSN: 1466-5026
Popis: A strictly anaerobic arsenate-respiring bacterium isolated from a gold mine in Bendigo, Victoria, Australia, belonging to the genus Bacillus is described. Cells are Gram-positive, motile rods capable of respiring with arsenate and nitrate as terminal electron acceptors using a variety of substrates, including acetate as the electron donor. Reduction of arsenate to arsenite is catalysed by a membrane-bound arsenate reductase that displays activity over a broad pH range. Synthesis of the enzyme is regulated; maximal activity is obtained when the organism is grown with arsenate as the terminal electron acceptor and no activity is detectable when it is grown with nitrate. Mass of the catalytic subunit was determined to be approximately 87 kDa based on ingel activity stains. The closest phylogenetic relative, based on 16S rRNA gene sequence analysis, is Bacillus arseniciselenatis, but DNA–DNA hybridization experiments clearly show that strain JMM-4T represents a novel Bacillus species, for which the name Bacillus macyae sp. nov. is proposed. The type strain is JMM-4T (=DSM 16346T=JCM 12340T).
Databáze: OpenAIRE
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