Partial silencing of a hydroxy-methylglutaryl-CoA reductase-encoding gene in Trichoderma harzianum CECT 2413 results in a lower level of resistance to lovastatin and lower antifungal activity
| Autor: | Antonio Llobell, Fran González, Santiago Gutiérrez, Juan Antonio Vizcaíno, M.R. Hermosa, Rosa E. Cardoza, Enrique Monte |
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| Rok vydání: | 2007 |
| Předmět: |
Genes
Fungal Microbial Sensitivity Tests Reductase Microbiology Rhizoctonia Fungal Proteins chemistry.chemical_compound Fusarium Drug Resistance Fungal Ergosterol Antibiosis Gene expression Fusarium oxysporum Genetics medicine Gene Silencing Lovastatin Cloning Molecular Phylogeny Trichoderma biology Terpenes food and beverages Trichoderma harzianum biology.organism_classification Hydroxymethylglutaryl-CoA reductase Biochemistry chemistry HMG-CoA reductase biology.protein Hydroxymethylglutaryl CoA Reductases Hydroxymethylglutaryl-CoA Reductase Inhibitors medicine.drug |
| Zdroj: | Fungal Genetics and Biology. 44:269-283 |
| ISSN: | 1087-1845 |
| DOI: | 10.1016/j.fgb.2006.11.013 |
| Popis: | In the present article, we describe the cloning and characterization of the Trichoderma harzianum hmgR gene encoding a hydroxymethylglutaryl CoA reductase (HMGR), a key enzyme in the biosynthesis of terpene compounds. In T. harzianum , partial silencing of the hmgR gene gave rise to transformants with a higher level of sensitivity to lovastatin, a competitive inhibitor of the HMGR enzyme. In addition, these hmgR -silenced transformants produced lower levels of ergosterol than the wild-type strain in a minimal medium containing lovastatin. The silenced transformants showed a decrease in hmgR gene expression (up to a 8.4-fold, after 72 h of incubation), together with an increase in the expression of erg7 (up to a 15.8-fold, after 72 h of incubation), a gene involved in the biosynthesis of triterpenes. Finally, hmgR -silenced transformants showed a reduction in their antifungal activity against the plant-pathogen fungi Rhizoctonia solani and Fusarium oxysporum . |
| Databáze: | OpenAIRE |
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