Ultraviolet light A irradiation induces immunosuppression associated with the generation of reactive oxygen species in human neutrophils
| Autor: | C. Li, Leiting Pan, Xinglei Su, Pengchong Jiang, Guangxue Xu, Mincai Chen, Xuechen Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Phagocytosis Biomedical Engineering Medicine (miscellaneous) Biology medicine.disease_cause Blood irradiation therapy lcsh:Technology 03 medical and health sciences 0302 clinical medicine Immune system UVA irradiation medicine Ultraviolet light lcsh:QC350-467 NAD(P)H chemistry.chemical_classification reactive oxygen species Reactive oxygen species immunosuppression lcsh:T Immunosuppression Atomic and Molecular Physics and Optics In vitro Electronic Optical and Magnetic Materials Cell biology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Immunology Human neutrophils sense organs Oxidative stress lcsh:Optics. Light |
| Zdroj: | Journal of Innovative Optical Health Sciences, Vol 9, Iss 1, Pp 1650001-1-1650001-7 (2016) |
| ISSN: | 1793-7205 1793-5458 |
| DOI: | 10.1142/S1793545816500012 |
| Popis: | Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspecific diseases in clinics; however, the underlying mechanisms remain largely unclear. Neutrophils, the first line of host defense, play a crucial role in a variety of inflammatory responses. In the present work, we investigated the effects of ultraviolet light A (UVA) on the immune functions of human neutrophils at the single-cell level by using an inverted fluorescence microscope. N-Formyl-methionyl-leucyl-phenylalanine (FMLP), a classic physiological chemotactic peptide, was used to induce a series of immune responses in neutrophils in vitro. FMLP-induced calcium mobilization, migration, and phagocytosis in human neutrophils was significantly blocked after treatment with 365[Formula: see text]nm UVA irradiation, demonstrating the immunosuppressive effects of UVA irradiation on neutrophils. Similar responses were also observed when the cells were pretreated with H2O2, a type of reactive oxygen species (ROS). Furthermore, UVA irradiation resulted in an increase in NAD(P)H, a member of host oxidative stress in cells. Taken together, our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils. |
| Databáze: | OpenAIRE |
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