FUNCTIONAL CHARACTERIZATION OF AN LCCL–LECTIN DOMAIN CONTAINING PROTEIN FAMILY IN PLASMODIUM BERGHEI
| Autor: | Daniel J. Carucci, Anthony A. Holder, John Yates, J. Dale Raine, Jean Langhorne, Isabelle Delrieu, Jeffrey R. Johnson, Laurence Florens, Robert E. Sinden, Holly E. Trueman, Johannes T. Dessens, Cecilia C. Waller, Jacqui Mendoza |
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| Rok vydání: | 2004 |
| Předmět: |
Protein family
Plasmodium berghei Blotting Western Molecular Sequence Data Protein domain Mutant Protozoan Proteins Gene Expression Polymerase Chain Reaction Plasmodium Apicomplexa Mice Lectins Anopheles parasitic diseases Animals Gene family Microscopy Phase-Contrast Amino Acid Sequence Fluorescent Antibody Technique Indirect Peptide sequence Ecology Evolution Behavior and Systematics biology biology.organism_classification Molecular biology Cell biology Multigene Family Female Parasitology |
| Zdroj: | Journal of Parasitology. 90:1062-1071 |
| ISSN: | 1937-2345 0022-3395 |
| Popis: | Using bioinformatic, proteomic, immunofluorescence, and genetic cross methods, we have functionally characterized a family of putative parasite ligands as potential mediators of cell-cell interactions. We name these proteins the Limulus clotting factor C, Coch-5b2, and Lgl1 (LCCL)-lectin adhesive-like protein (LAP) family. We demonstrate that this family is conserved amongst Plasmodium spp. It possesses a unique arrangement of adhesive protein domains normally associated with extracellular proteins. The proteins are expressed predominantly, though not exclusively, in the mosquito stages of the life cycle. We test the hypothesis that these proteins are surface proteins with 1 member of this gene family, lap1, and provide evidence that it is expressed on the surface of Plasmodium berghei sporozoites. Finally, through genetic crosses of wild-type Pblap1+ and transgenic Pblap1- parasites, we show that the null phenotype previously reported for sporozoite development in a Pblap1- mutant can be rescued within a heterokaryotic oocyst and that infectious Pblap1 sporozoites can be formed. The mutant is not rescued by coparasitization of mosquitoes with a mixture Pblap1+ and Pblap1- homokaryotic oocysts. |
| Databáze: | OpenAIRE |
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