Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean
| Autor: | Estela de Oliveira Nunes, Moisés de Aquino, Michael A. Birkett, Salvador Lima Brito Júnior, Clara Beatriz Hoffmann Campo, Francismar Corrêa Marcelino-Guimarães, Ricardo Vilela Abdelnor, Kenia de Carvalho, John A. Pickett, Talitta Regina Parmezan |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine glyceollin Glycine max QH426-470 Plant Genetics 01 natural sciences Terpene Transcriptome 03 medical and health sciences chemistry.chemical_compound Genetics Plant defense against herbivory Molecular Biology Gene Glyceollin biology Terpenes fungi food and beverages biology.organism_classification Terpenoid 030104 developmental biology Biochemistry chemistry Rust Phakopsora pachyrhizi Mevalonate pathway terpenes 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Biology v.43 n.4 2020 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 43, Issue: 4, Article number: e20190388, Published: 06 NOV 2020 Genetics and Molecular Biology, Vol 43, Iss 4 (2020) |
| ISSN: | 1678-4685 |
| Popis: | Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean. |
| Databáze: | OpenAIRE |
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