Minocycline modulates NFκB phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells
Autor: | Warren E. Rose, Alberto Daniel Guerra, W. John Kao, Peiman Hematti |
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Rok vydání: | 2017 |
Předmět: |
Adult
Male 0301 basic medicine MAPK/ERK pathway Staphylococcus aureus Stromal cell medicine.medical_treatment media_common.quotation_subject Mesenchymal stromal cells Medicine (miscellaneous) Minocycline Pharmacology Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Biomaterials lcsh:Biochemistry 03 medical and health sciences Western blot medicine Humans Animal model lcsh:QD415-436 Phosphorylation Internalization media_common lcsh:R5-920 medicine.diagnostic_test Chemistry Research Mesenchymal stem cell NF-kappa B Cell Biology Cells Immobilized Hydrogel 030104 developmental biology Cytokine Mesenchymal stem cells Molecular Medicine Female Antimicrobial lcsh:Medicine (General) Wound healing NFκB medicine.drug |
Zdroj: | Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-15 (2017) Stem Cell Research & Therapy |
ISSN: | 1757-6512 |
Popis: | Background Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties due to their anti-inflammatory, angiogenic, and even antibacterial properties. We have shown previously that minocycline enhances the wound healing phenotype of MSCs, and MSCs encapsulated in poly(ethylene glycol) and gelatin-based hydrogels with minocycline have antibacterial properties against Staphylococcus aureus (SA). Here, we investigated the signaling pathway that minocycline modulates in MSCs which results in their enhanced wound healing phenotype and determined whether preconditioning MSCs with minocycline has an effect on antimicrobial activity. We further investigated the in-vivo antimicrobial efficacy of MSC and antibiotic-loaded hydrogels in inoculated full-thickness cutaneous wounds. Methods Modulation of cell signaling pathways in MSCs with minocycline was analyzed via western blot, immunofluorescence, and ELISA. Antimicrobial efficacy of MSCs pretreated with minocycline was determined by direct and transwell coculture with SA. MSC viability after SA coculture was determined via a LIVE/DEAD® stain. Internalization of SA by MSCs pretreated with minocycline was determined via confocal imaging. All protein and cytokine analysis was done via ELISA. The in-vivo antimicrobial efficacy of MSC and antibiotic-loaded hydrogels was determined in Sprague–Dawley rats inoculated with SA. Two-way ANOVA for multiple comparisons was used with Bonferroni test assessment and an unpaired two-tailed Student’s t test was used to determine p values for all assays with multiple or two conditions, respectively. Results Minocycline leads to the phosphorylation of transcriptional nuclear factor-κB (NFκB), but not c-Jun NH2-terminal kinase (JNK) or mitogen-activated protein kinase (ERK). Inhibition of NFκB activation prevented the minocycline-induced increase in VEGF secretion. Preconditioning of MSCs with minocycline led to a reduced production of the antimicrobial peptide LL-37, but enhanced antimicrobial activity against SA via an increased production of IL-6 and SA internalization. MSC and antibiotic-loaded hydrogels reduced SA bioburden in inoculated wounds over 3 days and accelerated reepithelialization. Conclusions Minocycline modulates the NFκB pathway in MSCs that leads to an enhanced production of IL-6 and internalization of SA. This mechanism may have contributed to the in-vivo antibacterial efficacy of MSC and antibiotic-loaded hydrogels. Electronic supplementary material The online version of this article (doi:10.1186/s13287-017-0623-1) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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