Creatine treatment promotes differentiation of GABA-ergic neuronal precursors in cultured fetal rat spinal cord

Autor: Janine-Ai Schläppi, Theo Wallimann, Hans Rudolf Widmer, Rachel Qualls, Angélique Ducray, Robert H. Andres, Rolf W. Seiler, Uwe Schlattner
Přispěvatelé: Department of Neurosurgery, Université de Berne, Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Department of Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)-Institute of Cell Biology, Universität Bern [Bern] (UNIBE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF), Hamant, Sarah
Jazyk: angličtina
Rok vydání: 2007
Předmět:
MESH: Neurons
Tetrazolium Salts
MESH: Tubulin
MESH: gamma-Aminobutyric Acid
MESH: Spinal Cord
chemistry.chemical_compound
0302 clinical medicine
MESH: Pregnancy
Pregnancy
Tubulin
MESH: Propionic Acids
MESH: Animals
Creatine Kinase
Cells
Cultured
gamma-Aminobutyric Acid
Mitogen-Activated Protein Kinase 1
Neurons
MESH: Choline O-Acetyltransferase
0303 health sciences
MESH: Creatine Kinase
biology
MESH: Creatine
Kinase
Stem Cells
MESH: Energy Metabolism
Cell Differentiation
MESH: Tetrazolium Salts
Nitro Compounds
Immunohistochemistry
Choline acetyltransferase
MESH: Nitro Compounds
3. Good health
medicine.anatomical_structure
Spinal Cord
MESH: Cell Survival
Female
MESH: Cells
Cultured
MESH: Mitogen-Activated Protein Kinase 1
MESH: Cell Differentiation
medicine.medical_specialty
Neurite
MESH: Rats
Cell Survival
Blotting
Western
MESH: Thiazoles
MESH: Stem Cells
Creatine
Neuroprotection
Choline O-Acetyltransferase
Phosphocreatine
03 medical and health sciences
Cellular and Molecular Neuroscience
Internal medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
medicine
Animals
MESH: Blotting
Western
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
030304 developmental biology
MESH: Immunohistochemistry
Rats
Thiazoles
Endocrinology
chemistry
nervous system
biology.protein
Creatine kinase
Neuron
Propionates
Energy Metabolism
MESH: Female
030217 neurology & neurosurgery
Zdroj: Journal of Neuroscience Research
Journal of Neuroscience Research, Wiley, 2007, 85 (9), pp.1863-75. ⟨10.1002/jnr.21337⟩
ISSN: 0360-4012
1097-4547
DOI: 10.1002/jnr.21337⟩
Popis: International audience; Creatine is a substrate of cytosolic and mitochondrial creatine kinases. Its supplementation augments cellular levels of creatine and phosphocreatine, the rate of ATP resynthesis, and improves the function of the creatine kinase energy shuttle. High cytoplasmatic total creatine levels have been reported to be neuroprotective by inhibiting apoptosis. In addition, creatine has direct antioxidant effects, which may be of importance in amyotrophic lateral sclerosis. In the present study, we investigated the effects of creatine [5 mM] on survival and differentiation of cultured GABA-immunoreactive (-ir) and choline acetyltransferase (ChAT)-ir rat spinal cord neurons. Furthermore, we addressed the neuroprotective potential of creatine supplementation against 3-nitropropionic acid (3-NP) induced toxicity. General cell survival and total neuronal cell density were not altered by chronic creatine treatment. We found, however, after chronic creatine and short-term creatine exposure a significantly higher density of GABA-ir neurons hinting to a differentiation-inducing mechanism of creatine. This notion is further supported by a significant higher content of GAD after creatine exposure. Creatine supplementation also exerted a partial, but significant neuroprotection for GABA-ir neurons against 3-NP induced toxicity. Interestingly, chronic creatine treatment did not alter cell density of ChAT-ir neurons but promoted their morphologic differentiation. Cell soma size and number of primary neurites per neuron were increased significantly after creatine supplementation. Taken together, creatine supplementation promoted the differentiation or the survival of GABAergic neurons and resulted in partial neuroprotection against 3-NP induced toxicity. The data suggest that creatine may play a critical role during development of spinal cord neurons.
Databáze: OpenAIRE
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