Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice
Autor: | Amir Maksyutov, Valeriy Tereshchenko, Nadezda Knauer, Julia A. Lopatnikova, Sergey Sennikov, Julia Nikolaevna Khantakova, Julia Shevchenko, Maria Kuznetsova, V. V. Kurilin, Alexander N. Silkov, Aleksey S Bulygin |
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Rok vydání: | 2020 |
Předmět: |
Graft Rejection
C57BL/6 Article Subject Immunology Graft vs Host Disease chemical and pharmacologic phenomena Transfection Major histocompatibility complex Immune tolerance Epitopes Mice 03 medical and health sciences 0302 clinical medicine Antigen T-Lymphocyte Subsets Gene Order Immune Tolerance Animals Transplantation Homologous Immunology and Allergy 030304 developmental biology 0303 health sciences biology H-2 Antigens FOXP3 Interleukin Dendritic Cells General Medicine RC581-607 biology.organism_classification Mice Inbred C57BL Transplantation Disease Models Animal 030220 oncology & carcinogenesis Mice Inbred CBA biology.protein Female Immunologic diseases. Allergy Plasmids Research Article |
Zdroj: | Journal of Immunology Research, Vol 2020 (2020) Journal of Immunology Research |
ISSN: | 2314-7156 2314-8861 |
Popis: | Background. Nonspecific immunosuppressive therapy for graft rejection and graft-versus-host disease (GVHD) is often accompanied by severe side effects such as opportunistic infections and cancers. Several approaches have been developed to suppress transplantation reactions using tolerogenic cells, including induction of FoxP3+ Tregs with antigen-loaded dendritic cells (DCs) and induction of CD4+IL-10+ cells with interleukin IL-10-producing DCs. Here, we assessed the effectiveness of both approaches in the suppression of graft rejection and GVHD. Methods. IL-10-producing DCs were generated by the transfection of DCs with DNA constructs encoding mouse IL-10. Antigen-loaded DCs from C57BL/6 mice were generated by transfection with DNA constructs encoding antigenic determinants from the H2 locus of CBA mice which differ from the homologous antigenic determinants of C57BL/6 mice. Results. We found that both IL-10-producing DCs and antigen-loaded immature DCs could suppress graft rejection and GVHD but through distinct nonspecific and antigen-specific mechanisms, respectively. Discussion. We provide data that the novel approach for DCs antigen loading using DNA constructs encoding distinct homologous determinants derived from major histocompatibility complex genes is effective in antigen-specific suppression of transplantation reactions. Such an approach eliminates the necessity of donor material use and may be useful in immunosuppressive therapy side effects prevention. |
Databáze: | OpenAIRE |
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